Adhesion force, neutrophil membrane stiffness and systemic inflammation biomarkers in patients with chronic obstructive pulmonary disease and pulmonary hypertension
https://doi.org/10.18093/0869-0189-2014-0-6-33-39
Abstract
The aim of this study was to investigate blood neutrophil functional properties and the relationships to systemic inflammation in order to better understand a role of systemic inflammation for PH occurrence in COPD patients. Methods. This was a local open comparative study involving 30 patients with COPD (15 patients with PH and 15 patients without PH). The blood neutrophil functional properties (cell membrane stiffness and adhesion force) were measured using the atomic force microscopy. The power spectroscopy was used to quantitatively assess the adhesion force and neutrophil cell membrane stiffness (the Young's modulus). Results. Blood neutrophil and thrombocyte numbers, CRP and fibrinogen levels were significantly higher in patients with COPD + PH compared to COPD patients without PH. Relationships were found between systemic inflammation biomarkers and systolic pulmonary artery pressure. Conclusion. The results demonstrated that the membrane stiffness and the adhesion force were significantly increased in COPD patients with PH compared to controls and to COPD patients without PH. These neutrophil parameters were closely related to systemic inflammation.
About the Authors
V. V. Gaynitdinova
State Institution "Bashkir State Medical University": 3, Lenina ul., Ufa, 3450000, Bashkir Republic, Russia
Russian Federation
Russian Federation
PhD, Associate Professor, Chair of Therapy and Clinical Pharmacology, State Budget High-Level Educational Institution "Bashkir State University"; tel.: (347) 272-23-85
L. A. Sharafutdinova
State Institution "Bashkir State University", The Center of Nano- and Microdynamics of Disperse Systems: 32, Z.Validi ul., Ufa, 450076, Bashkir Republic, Russia
Russian Federation
Russian Federation
PhD in Biology, Associate Professor, Chair of Human Physiology and Zoology, Biological Faculty, State Budget High-Level Educational Institution "Bashkir State University"; tel.: (347) 229-96-71
I. M. Kamaltdinov
State Institution "Bashkir State University", The Center of Nano- and Microdynamics of Disperse Systems: 32, Z.Validi ul., Ufa, 450076, Bashkir Republic, Russia
Russian Federation
Russian Federation
PhD in Technics, Research Technician, The Center of Nanodynamics and Microdynamics of Disperse Systems, State Budget High-Level Educational Institution "Bashkir State University"; tel.: (347) 229-97-26
S. N. Avdeev
Federal Institution "Pulmonology Research Institute", Federal Medical and Biological Agency of Russia: 32, build. 4, 11th Parkovaya ul., Moscow, 105077, Russia
Russian Federation
Russian Federation
MD, Professor, Head of Clinical Department, Federal State Budget institution "Pulmonology Research Institute", Federal Medical and Biological Agency of Russia, Moscow, Russia; tel.: (495) 465-52-64
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Review
For citations:
Gaynitdinova V.V., Sharafutdinova L.A., Kamaltdinov I.M., Avdeev S.N. Adhesion force, neutrophil membrane stiffness and systemic inflammation biomarkers in patients with chronic obstructive pulmonary disease and pulmonary hypertension. PULMONOLOGIYA. 2014;(6):33-39. (In Russ.) https://doi.org/10.18093/0869-0189-2014-0-6-33-39
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