pulmoПульмонологияPULMONOLOGIYA0869-01892541-9617Scientific and Practical Journal “PULMONOLOGIYA” LLC10.18093/0869-0189-2014-0-6-33-39pulmo-490Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL STUDIESСила адгезии, жесткость мембраны нейтрофилов и биомаркеры системного воспаления у больных хронической обструктивной болезнью легких с легочной гипертензиейAdhesion force, neutrophil membrane stiffness and systemic inflammation biomarkers in patients with chronic obstructive pulmonary disease and pulmonary hypertensionГайнитдиноваВ. В.GaynitdinovaV. V.

к. м. н., доцент кафедры терапии и клинической фармакологии ИПО ГБОУ ВПО "БашГУ" Минздрава России; тел.: (347) 272-23-85

PhD, Associate Professor, Chair of Therapy and Clinical Pharmacology, State Budget High-Level Educational Institution "Bashkir State University"; tel.: (347) 272-23-85

ivv_08@mail.ruШарафутдиноваЛ. А.SharafutdinovaL. A.

к. б. н., доцент кафедры физиологии человека и зоологии биологического факультета ГБОУ ВПО "БашГУ"; тел.: (347) 229-96-71

PhD in Biology, Associate Professor, Chair of Human Physiology and Zoology, Biological Faculty, State Budget High-Level Educational Institution "Bashkir State University"; tel.: (347) 229-96-71

sharafla@yandex.ruКамалтдиновИ. М.KamaltdinovI. M.

к. т. н., лаборант-исследователь Центра микрои наномасштабной динамики дисперсных систем ГБОУ ВПО "БашГУ"; тел.: (347) 229-97-26

PhD in Technics, Research Technician, The Center of Nanodynamics and Microdynamics of Disperse Systems, State Budget High-Level Educational Institution "Bashkir State University"; tel.: (347) 229-97-26

kamil@fizfaka.netАвдеевС. Н.AvdeevS. N.

д. м. н., профессор, руководитель клинического отдела ФГБУ "НИИ пульмонологии" ФМБА России; тел.: (495) 465-52-64

MD, Professor, Head of Clinical Department, Federal State Budget institution "Pulmonology Research Institute", Federal Medical and Biological Agency of Russia, Moscow, Russia; tel.: (495) 465-52-64

serg_avdeev@list.ruГБОУ ВПО "Башкирский государственный медицинский университет" Минздрава России: 450000, Россия, Уфа, ул. Ленина, 3;РоссияState Institution "Bashkir State Medical University": 3, Lenina ul., Ufa, 3450000, Bashkir Republic, RussiaRussian FederationГБОУ ВПО "Башкирский государственный университет", Центр микро- и наномасштабной динамики дисперсных систем: 450076, Россия, Уфа, ул. З.Валиди, 32РоссияState Institution "Bashkir State University", The Center of Nano- and Microdynamics of Disperse Systems: 32, Z.Validi ul., Ufa, 450076, Bashkir Republic, RussiaRussian FederationФГБУ "НИИ пульмонологии" ФМБА России: 105077, Москва, ул. 11-я Парковая, 32, корп. 4РоссияFederal Institution "Pulmonology Research Institute", Federal Medical and Biological Agency of Russia: 32, build. 4, 11th Parkovaya ul., Moscow, 105077, RussiaRussian Federation201428122014063339Copyright © Гайнитдинова В.В., Шарафутдинова Л.А., Камалтдинов И.М., Авдеев С.Н., 20142014Гайнитдинова В.В., Шарафутдинова Л.А., Камалтдинов И.М., Авдеев С.Н.Gaynitdinova V.V., Sharafutdinova L.A., Kamaltdinov I.M., Avdeev S.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.pulmonology.ru/pulm/article/view/490

Методом атомно-силовой микроскопии проведен анализ вязкоупругих свойств (силы адгезии и жесткости мембраны) нейтрофилов, изучены их взаимосвязи с биомаркерами системного воспаления (СВ) у больных хронической обструктивной болезнью легких(ХОБЛ) в зависимости от наличия легочной гипертензии (ЛГ). В режиме силовой спектроскопии определена сила адгезии и количественная оценка жесткости (модуля Юнга) клеточной мембраны нейтрофилов. По результатам исследования показано, что у больных ХОБЛ + ЛГ наблюдается значимое увеличение жесткости мембраны (повышение значения модуля Юнга) и силы адгезии нейтрофилов по сравнению с контролем и больными без ЛГ. Показана тесная взаимосвязь исследуемых показателей нейтрофилов и биомаркеров СВ. 

The aim of this study was to investigate blood neutrophil functional properties and the relationships to systemic inflammation in order to better understand a role of systemic inflammation for PH occurrence in COPD patients. Methods. This was a local open comparative study involving 30 patients with COPD (15 patients with PH and 15 patients without PH). The blood neutrophil functional properties (cell membrane stiffness and adhesion force) were measured using the atomic force microscopy. The power spectroscopy was used to quantitatively assess the adhesion force and neutrophil cell membrane stiffness (the Young's modulus). Results. Blood neutrophil and thrombocyte numbers, CRP and fibrinogen levels were significantly higher in patients with COPD + PH compared to COPD patients without PH. Relationships were found between systemic inflammation biomarkers and systolic pulmonary artery pressure. Conclusion. The results demonstrated that the membrane stiffness and the adhesion force were significantly increased in COPD patients with PH compared to controls and to COPD patients without PH. These neutrophil parameters were closely related to systemic inflammation.

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The authors declare that there are no conflicts of interest present.