Changes in nitric oxide metabolism in co-morbidity of chronic obstructive pulmonary disease and chronic cerebral ischemia

Summary. The study was aimed at investigation of nitric oxide (NO) metabolism including total and separate concentrations of stable NO metabolites (nitrate anions and nitrite anions) and 3-nitrotirosine concentration in stable chronic obstructive pulmonary disease (COPD) and in COPD with concomitant chronic cerebral ischemia (CCI). The study involved 55 patients aged 51 to 67 years divided into 2 groups: 28 patients with COPD II–III stage (the 1st group) and 27 patients with COPD II–III stage and concomitant CCI II–III stage (the 2nd group). The control group included 25 healthy non-smoking volunteers. A significant increase in nitrate anion concentration and in the total nitrite and nitrate concentration was found in the blood of patients of both groups compared to the controls. A statistically significant inverse relationship was found between the blood nitrite anion concentration and FEV1 (r = –0.77; p < 0.05) in COPD patients. These results demonstrate that changes in NO metabolism related to nitrous stress could emerge even in stable COPD. In co-existing COPD and CCI, changes in these biomarkers could be also related to functional particularities of nitrite reductase apart from the nitrous stress.

1. Global Initiative for Chronic Obstructive Lung Disease. Global strategy for the diagnosis, management, and prevention of chronic obstructive lung disease. Update 2009. Medical communications resources, Inc.; 2009 (10).

2. Безлепко А.В. Захарова П.К., Безлепко Е.А. Сопутствующие заболевания у больных ХОБЛ. В кн.: Сборник трудов XX Национального конгресса по болезням органов дыхания, М.; 2010. 442–443.

3. Ванин А.Ф. Оксид азота в биологии: история, состояние и перспективы исследований. Биохимия 1998; 63 (7): 867–869.

4. Сосунов A.A. Оксид азота как межклеточный посредник. Соросов. образоват. журн. 2000; 12: 27–34.

5. Ванин А.Ф. Оксид азота в биомедицинских исследованиях. Вестн. РАМН 2000; 4: 3–5.

6. Cookson W. Polygenes, asthma, and atopy. In: From Genetics to quality of life: XV World congress of asthmology. Seattle; 1996. 15–19.

7. Raychaudhuri B., Dweik K., Connors M.J. et al. Nitric oxide blocks nuclear factor-kappa B activation in alveolar macrophages. Am. J. Respir. Cell. Mol. Biol. 1999; 21 (3): 311–316.

8. Carratu P., Scoditti C., Maniscalco M. Exhaled and arterial levels of endothelin-1 are increased and correlate with pulmonary systolic pressure in COPD with pulmonary hypertension. BMC Pulm. Med. 2008; 8 (5): 154–158.

9. Corradi M., Pesci A., Casana R. Nitrate in exhaled breath condensate of patients with different airway diseases. Nitric Oxide 2003; 8 (1): 26–30.

10. Barnes P.J., Dweik R.A., Gelb A.F. et al. Exhaled nitric oxide in pulmonary diseases: a comprehensive review. Chest 2010; 138: 682–692.

11. Blake M.J., Klevay L.M., Halas E.S., Bode A.M. Blood pressure and heat shock protein expression in response to acute and chronic stress. Hypertension 1995; 25 (1): 539–544.

12. Нечипуренко Н.И., Пашковская И.Д., Мусиенко Ю.И. Основные патофизиологические механизмы ишемии головного мозга. Мед. новости 2008; 1: 7–13.

13. Davis K.L., Martin E., Turko I.V., Murad F. Novel effects of nitric oxide. Annu. Rev. Pharmacol. Toxicol. 2001; 41: 203–236.

14. Debon P. C., Tannenbaum S. R. Reactive nitrogen species in the chemical biology of inflammation. Arch. Biochem. Biophys. 2004; 423 (1): 12–22.

15. Климанов И.А. Изучение метаболизма оксида азота при бронхиальной астме: Автореф. дис. … канд. мед. наук. М.; 2006.

16. Реутов В.П., Сорокина Е.Г., Охотин В.Е., Косицин Н.С. Циклические превращения оксида азота в организме млекопитающих. М.: Наука; 1998.