Relationship between chemiluminescent and enzymatic activity of blood neutrophils interactions in patients with community6 acquired pneumonia
https://doi.org/10.18093/0869-0189-2015-25-6-685-692
Abstract
The aim of the study was to investigate enzymatic and chemiluminescent activity in blood neutrophils of patients with moderate and severe communityacquired pneumonia (CAP).
Methods. The study involved 48 patients with moderate CAP and 40 patients with severe CAP; they were examined on admission. Synthesis of primary and secondary reactive oxygen species (ROS) was evaluated using the chemiluminescence assay. Neutrophil NAD(P)dependent dehydrogenase activity was assessed using the bioluminescent method.
Results. The «respiratory burst» was found in neutrophils of CAP patients. Terminal reactions of the anaerobic glycolysis and the tricarbonic acid cycle were intensified in patients with severe CAP only. Increased lipid anabolism activity and substrate outflow from the tricarbonic acid cycle via NADHdependent glutamate dehydrogenase were shown regardless of CAP severity. The canonical analysis showed abnormalities of metabolic mechanisms of the «respiratory burst» in blood neutrophils in CAP.
Conclusion. The metabolic disorders and «respiratory burst» intensity in CAP arize the need for pathogenic correction. High NADHdepend ent dehydrogenase activity and lower chemiluminescent activity in blood neutrophils could be used as additional markers of severe course of CAP.
About the Authors
A. A. Savchenko
Federal Research Institute of Medical Problems of the North, 3g, Partizana Zheleznyaka str., Krasnoyarsk, 660022, Russia
V.F.Voyno-Yasenetskiy Krasnoyarsk State Medical University, Healthcare Ministry of Russia; 1, Partizana Zheleznyaka str., Krasnoyarsk, 660022, Russia
Russian Federation
Russian Federation
MD, Professor, Head of Laboratory of Molecular Physiology and Pathology, Federal Research Institute of Medical Problems of the North; Head of A.T.Pshonik Department of Physiology, V.F.VoynoYasenetskiy Krasnoyarsk State Medical University, Healthcare Ministry of Russia; tel.: (391) 2125263, fax: (391) 2280683
Yu. I. Grinshteyn
V.F.Voyno-Yasenetskiy Krasnoyarsk State Medical University, Healthcare Ministry of Russia; 1, Partizana Zheleznyaka str., Krasnoyarsk, 660022, Russia
Russian Federation
Russian Federation
MD, Professor, Head of Department of Therapy, Physician Postgraduate Education Faculty, V.F.VoynoYasenetskiy Krasnoyarsk State Medical University, Healthcare Ministry of Russia; tel.: (391) 2424664
L. B. Dresvyankina
Federal Research Institute of Medical Problems of the North, 3g, Partizana Zheleznyaka str., Krasnoyarsk, 660022, Russia
Russian Federation
Russian Federation
Junior Researcher at Laboratory of Molecular Physiology and Pathology, Federal Research Institute of Medical Problems of the North; V.F.VoynoYasenetskiy Krasnoyarsk State Medical University, Healthcare Ministry of Russia; tel.: (391) 2125263, fax: (391) 2280683
A. I. Aristov
V.F.Voyno#Yasenetskiy Krasnoyarsk State Medical University, Healthcare Ministry of Russia; 1, Partizana Zheleznyaka str., Krasnoyarsk, 660022, Russia
Russian Federation
Russian Federation
ассистент кафедры терапии ИПО ГБОУ ВПО «Красноярский государственный медицинский университет им. проф. В.Ф.ВойноЯсенецкого» Минздрава России; тел.: (391) 295 5226
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Review
For citations:
Savchenko A.A., Grinshteyn Yu.I., Dresvyankina L.B., Aristov A.I. Relationship between chemiluminescent and enzymatic activity of blood neutrophils interactions in patients with community6 acquired pneumonia. PULMONOLOGIYA. 2015;25(6):685-692. (In Russ.) https://doi.org/10.18093/0869-0189-2015-25-6-685-692
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