ATP-degrading activity membranes from lung tissue: peculiarities of hydrolisis cellular specificity and localization
Abstract
Peculiarities of ATP and ADP degradation by lung membranes were studed. It was shown that AMP is the final product of ATP hydrolysis. AMP is formed from ATP by sequential release of terminal phosphate according to the scheme: ATP─ ─→ADP─ ─→AMP, and not by pyrophosphatase activity.
Inhibitory analysis using adenine nucleotides, their nonhydrolysible analogs, phosphate and pyrophosphate and also determination of kinetic values (Kni and Vmax) for the ATP-ase and ADP-ase reactions indicates on a coordinated hydrolysis of ATP and ADP.
ATP-degradating activity is discovered in membranes of cells formed a blood vessels (endothelial and smooth-muscle), but not in membranes of Type II alveolar cells, which secrete surfactant.
This nucleotide hydrolising activity is preferentially localised on a luminal surfase of plasma membrane, since addition of a pore-forming antibiotic alameticine to a confluent endothelial monolayer does not significantly increase the ATP hydrolysis.
About the Authors
E. V. Nupenko
Кардиологический научный центр России
Russian Federation
Russian Federation
M. P. Panchenko
Кардиологический научный центр России
Russian Federation
Russian Federation
Yu. A. Romanov
Кардиологический научный центр России
Russian Federation
Russian Federation
A. V. Grishin
Кардиологический научный центр России
Russian Federation
Russian Federation
N. V. Kabaeva
Кардиологический научный центр России
Russian Federation
Russian Federation
A. S. Antonov
Кардиологический научный центр России
Russian Federation
Russian Federation
V. A. Tkachuk
Кардиологический научный центр России
Russian Federation
Russian Federation
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Review
For citations:
Nupenko E.V., Panchenko M.P., Romanov Yu.A., Grishin A.V., Kabaeva N.V., Antonov A.S., Tkachuk V.A. ATP-degrading activity membranes from lung tissue: peculiarities of hydrolisis cellular specificity and localization. PULMONOLOGIYA. 1992;(4):53-60. (In Russ.)
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