VIRULENT PROPERTIES OF GENUS CANDIDA IN PATIENTS WITH PERSISTENT BRONCHIAL ASTHMA AND LONG-TERM TREATMENT WITH INHALED STEROIDS
https://doi.org/10.18093/0869-0189-2014-0-3-73-77
Abstract
Aim. The aim of this study was to investigate virulent properties of the genus Candida spp. in patients with persistent bronchial asthma and long-term treatment with inhaled steroids.
Methods. In this study, virulent properties of the genus Candida spp. were investigated in oropharyngeal smears of 30 patients with persistent asthma treated with moderate-to-high dose inhaled corticosteroids (ICS) for at least 1 year. A control group included 15 steroid-naive asthmatic patients. Fungi were identified by conventional methods (morphology, presence of germination tubes, chlamydospores, fermentation of carbohydrates). Sensitivity to antibiotics was determined by disk method on the Saburo agar medium. Adhesion was assessed in a model with nitrocellulose film and immobilized hemoglobin. Proteinase activity was measured by the ELISA method in supernatant at two pH values: acidic (pH = 4.5) or weakly alkaline
(pH = 7.8). CD cell leukocyte receptor proteolysis was evaluated by the cytofluorometrics method.
Results. An incidence of fungal colonization of the pharynx in ICS-treated patients with bronchial asthma was 76.7 %. Most strains were highly virulent as they had significantly higher adhesive and enzymatic activity and multi-drug resistance to antifungals compared to control group.
Conclusion. The ability of these strains to secrete proteolytic enzymes against CD4+ T-lymphocyte surface receptors could provide an additional immunomodulatory mechanism in Candida albicans invasion.
Methods. In this study, virulent properties of the genus Candida spp. were investigated in oropharyngeal smears of 30 patients with persistent asthma treated with moderate-to-high dose inhaled corticosteroids (ICS) for at least 1 year. A control group included 15 steroid-naive asthmatic patients. Fungi were identified by conventional methods (morphology, presence of germination tubes, chlamydospores, fermentation of carbohydrates). Sensitivity to antibiotics was determined by disk method on the Saburo agar medium. Adhesion was assessed in a model with nitrocellulose film and immobilized hemoglobin. Proteinase activity was measured by the ELISA method in supernatant at two pH values: acidic (pH = 4.5) or weakly alkaline
(pH = 7.8). CD cell leukocyte receptor proteolysis was evaluated by the cytofluorometrics method.
Results. An incidence of fungal colonization of the pharynx in ICS-treated patients with bronchial asthma was 76.7 %. Most strains were highly virulent as they had significantly higher adhesive and enzymatic activity and multi-drug resistance to antifungals compared to control group.
Conclusion. The ability of these strains to secrete proteolytic enzymes against CD4+ T-lymphocyte surface receptors could provide an additional immunomodulatory mechanism in Candida albicans invasion.
About the Authors
I. D. Reshetnikova
Federal Institution Kazan Research Institute of Epidemiology and Microbiology; Kazan, Russia
Russian Federation
Russian Federation
Yu. A. Tyurin
Federal Institution Kazan Research Institute of Epidemiology and Microbiology; Kazan, Russia
State Institution Kazan State Medical University, the Health Ministry of Russia; Kazan, Russia
Russian Federation
Russian Federation
N. I. Glushko
Federal Institution Kazan Research Institute of Epidemiology and Microbiology; Kazan, Russia
Russian Federation
Russian Federation
S. A. Lisovskaya
Federal Institution Kazan Research Institute of Epidemiology and Microbiology; Kazan, Russia
Russian Federation
Russian Federation
I. G. Mustafin
State Institution Kazan State Medical University, the Health Ministry of Russia; Kazan, Russia
Russian Federation
Russian Federation
R. S. Fassakhov
Federal Institution Kazan Research Institute of Epidemiology and Microbiology; Kazan, Russia
Russian Federation
Russian Federation
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Review
For citations:
Reshetnikova I.D., Tyurin Yu.A., Glushko N.I., Lisovskaya S.A., Mustafin I.G., Fassakhov R.S. VIRULENT PROPERTIES OF GENUS CANDIDA IN PATIENTS WITH PERSISTENT BRONCHIAL ASTHMA AND LONG-TERM TREATMENT WITH INHALED STEROIDS. PULMONOLOGIYA. 2014;(3):73-77. (In Russ.) https://doi.org/10.18093/0869-0189-2014-0-3-73-77
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