TLR-4 GENE POLYMORPHISM (ASP299GLY) IN ADULT PATIENTS WITH ATOPIC AND NON-ATOPIC BRONCHIAL ASTHMA IN THE CRIMEA POPULATION
https://doi.org/10.18093/0869-0189-2014-0-3-68-72
Abstract
The aim of the study was to investigate TLR-4 gene polymorphism in adult patients with atopic and non-atopic bronchial asthma in the Crimea population.
Methods. TLR-4 gene polymorphism (Asp299Gly) was studied in 275 patients with atopic asthma and 56 patients with non-atopic asthma; a control group included 285 healthy residents of Crimea. The allele-specific polymerase chain reaction with electrophoretic detection of polymorphism was used.
Results. In the control group, genotype frequency distribution (AA 85 %, AG 14 %, GG 1 %) was significantly different (c2 = 6.598, p = 0.037) from that in the atopic asthmatics (AA 76 %, AG 22 %, GG 2 %). In patients with non-atopic asthma, genotype frequency distribution (AA 91 %, AG 9 %, GG 0 %) did not differ significantly (c2 = 1.721, p = 0.423) from that in the control group. The results have shown that the risk of atopic asthma development was associated with increase in G-allele and decrease in A allele frequency (OR = 1.634, c2 = 6.08, p = 0.014).
Conclusion. Thus, TLR-4 gene polymorphism (Asp299Gly) is related to occurrence of bronchial asthma in the Crimea population. The AA genotype (Asp299 Asp) of this gene plays a protective role against asthma occurrence. The G allele frequency in patients with atopic asthma was significantly higher than it was in patients with non-atopic asthma.
Methods. TLR-4 gene polymorphism (Asp299Gly) was studied in 275 patients with atopic asthma and 56 patients with non-atopic asthma; a control group included 285 healthy residents of Crimea. The allele-specific polymerase chain reaction with electrophoretic detection of polymorphism was used.
Results. In the control group, genotype frequency distribution (AA 85 %, AG 14 %, GG 1 %) was significantly different (c2 = 6.598, p = 0.037) from that in the atopic asthmatics (AA 76 %, AG 22 %, GG 2 %). In patients with non-atopic asthma, genotype frequency distribution (AA 91 %, AG 9 %, GG 0 %) did not differ significantly (c2 = 1.721, p = 0.423) from that in the control group. The results have shown that the risk of atopic asthma development was associated with increase in G-allele and decrease in A allele frequency (OR = 1.634, c2 = 6.08, p = 0.014).
Conclusion. Thus, TLR-4 gene polymorphism (Asp299Gly) is related to occurrence of bronchial asthma in the Crimea population. The AA genotype (Asp299 Asp) of this gene plays a protective role against asthma occurrence. The G allele frequency in patients with atopic asthma was significantly higher than it was in patients with non-atopic asthma.
About the Author
Yu. A. Bisyuk
State Institution S.I.Georgievskiy Crimea State Medical University; Simferopol, Russia
Russian Federation
Russian Federation
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Review
For citations:
Bisyuk Yu.A. TLR-4 GENE POLYMORPHISM (ASP299GLY) IN ADULT PATIENTS WITH ATOPIC AND NON-ATOPIC BRONCHIAL ASTHMA IN THE CRIMEA POPULATION. PULMONOLOGIYA. 2014;(3):68-72. (In Russ.) https://doi.org/10.18093/0869-0189-2014-0-3-68-72
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