The TaqI polymorphism in the 3’flanking region of the pi gene in the chronic pulmonary diseases and lung cancer

Abstract

The TaqI polymorphism in the 3’flanking region of the PI gene has been analyzed in 147 patients with different lung diseases and 110 healthy individuals. The detection of the TaqI polymorphism was carried out by a single step or two-step nested polymerase chain reaction followed by the TaqI digestion of the fmplfied product. The frequency of TaqI polymorphism was significantly higher among patients with chronic obstructive lung disease (15.15% versus 5.45%). Despite the difference between healthy controls and patients with lung cancer was nonsignificant, the X² value for this comparison was quite close to the level of statistical significance. The group of patients with other lung diseases did not reveal any difference from the control in the frequency of TaqI polimorphism.