Morphological functional structure of smooth muscular tissue for hum an bronchi of different generations
Abstract
Human bronchial smooth muscular cells isolated by the alkaline dissociation technique were studied. Morphometric indices of myocytes were specified, in addition the contents of both DNU in nuclei and totai cytoplasma protein were determined using scanning cytospectrophotometer. Subpopulations of small, mid and large leiomyocytes were also distinguished. It was shown that the mid volume of smooth muscular cells in large, mid and small bronchi differ reliably. With the bronchus diameter to decrease, the share of small myocytes increases and the subpopulation of large myocytes decreases in the structure of the muscular tissue population. Small myocytes are the most dynamic component of the cell’s muscular tissue population. Their prevalence is considered relevant to the high adaptation and functional ability of small bronchi.
About the Authors
A. L. Zashikhin
Кафедра гистологии, цитологии и эмбриологии Архангельской государственной медицинской академии;
лаборатория патоморфологии НИИ пульмонологии М3 РФ
Russian Federation
Russian Federation
Yu. V. Agafonov
Кафедра гистологии, цитологии и эмбриологии Архангельской государственной медицинской академии;
лаборатория патоморфологии НИИ пульмонологии М3 РФ
Russian Federation
Russian Federation
L. A. Zaostrovskaya
Кафедра гистологии, цитологии и эмбриологии Архангельской государственной медицинской академии;
лаборатория патоморфологии НИИ пульмонологии М3 РФ
Russian Federation
Russian Federation
A. L. Cherniayev
Кафедра гистологии, цитологии и эмбриологии Архангельской государственной медицинской академии;
лаборатория патоморфологии НИИ пульмонологии М3 РФ
Russian Federation
Russian Federation
V. P. Bykov
Кафедра гистологии, цитологии и эмбриологии Архангельской государственной медицинской академии;
лаборатория патоморфологии НИИ пульмонологии М3 РФ
Russian Federation
Russian Federation
References
1. Вейбель Э.Р. Морфометрия легких человека — М.: Медицина 1970.
2. Есипова И.К., Алексеевских Ю.Г. Структурно-функциональные особенности крупных и мелких бронхов и различия возникающих в них воспалительных реакций / / Арх. пат.— 1994 — № 4.— С.6.
3. Зашихин A.Л., Агафонов Ю.В. Динамика популяции мышечных клеток воздухоносных путей при изменении функциональной нагрузки легких / / Пульмонология.— 1994.— № 3.— С.34—41.
4. Непомнящих Г.И. Патологическая анатом ия и ультраструктура бронхов при хроническом воспалении легких.— Новосибирск: Наука, 1979.— 294 с.
5. Brown R.H., Herold C.J., Hirshman С.А., Zerhouni E.A., Mitzner W. Individual airway constriction response heterogeneity to histamine assessed by high-resolution computed tomography / / J. Appl Physiol.— 1993.— Vol.74.— P.2615—2620.
6. Chitano P., Sigurdsson S.B., Halayko A.J., Stephens N.L. Relevance of classification by size to topographical differences in bronchial smooth muscle response / / Ibid.— Vol.75.— P.2013—2021.
7. Ebina М., Yaegashi H., Takahashi Т., Motomiya М., Taneruma M. Distribution of smooth muscle along the bronchial tree. A morphometric study of ordinary autopsy lungs / / Am. Rev Respir. Dis.— 1990.— Vol.141.— P.1322—1326.
8. Ebina М., Takahashi Т., Chiba Т., Motomiya M. Cellular hypertrophy and hyperplasia of airway smooth muscle underlying bronchial asthma. A 3-D morphometric study / / Ibid.— 1993 — Vol. 148.— P.720—726.
9. Halayko A.J., Salari Y., Ma X., Stephenes N.L. Markers of airways smooth muscle cell phenotype / / Am. J. Physiol. Lung Cell. Mol. Physiol.— 1996.— Vol.270.— P.L1040—L1051.
10. Horsfield K. The Structure of the Trecheobronchial Tree / Ed. J.G.Scadding. G.Cumming, W.M.Thurlbeck.— London: Heinemann, 1981.— P.54—70.
11. James A.L., Pare P.D., Hogg J.C. The mechanics of airway narrowing in asthma / / Am. Rev. Respir. Dis.— 1989.— Vol.139.— P.242—246.
12. Ma Xuefei, Li W., Stephens N.L. Detection of two clusters of mechanical properties of smooth muscle along the airway tree / / J.Appi. Physiol.— 1996.— Vol.80, № 3.— P.857—861.
13. Mitchell R.W., Kelly E., Leff A.R. Reduced acetylcholinesterase in canine tracheal smooth muscle hom ogenates after active immune-sensitization / / Am. J. Respir. Cell Mol. Biol.— 1991.— Vol.5.— P.56—62.
14. Ohta K., Mortenson R.L., Clark R.A.F., Hirose N., King T.E.Jr. Immunohistochemicai identification and characterization of smooth muscle — like cells in idiopathic pulmonary fibrosis / / Am. J. Respir. Crit. Care Med.— 1995.— Vol.152.— P.1659—1665.
15. Zashikin A., Agafonov Y. Allergen-indused airway Hyperresponsiveness in guinea pigs: the characteristic feature of smooth muscle cell population / / Allergy.— 1996,— Vol.51, N° 32.— P.194.
Review
For citations:
Zashikhin A.L., Agafonov Yu.V., Zaostrovskaya L.A., Cherniayev A.L., Bykov V.P. Morphological functional structure of smooth muscular tissue for hum an bronchi of different generations. PULMONOLOGIYA. 1997;(3):61-64. (In Russ.)
Views:
630
Email this article 