The differences of characteristics of blood eosinophils in patients affected with bronchial asthma, atopic dermatitis and opisthorchiasis
Abstract
E-rosette formation by low-density (LDE) and high-density (HDE) eosinophils was studied in sheep erythrocytes. The group of patients revealed a 4— 17 times greater quantity of LDE than the healthy group. HDE demonstrated a much smaller difference. The very high rosette-forming capacity of LDE in the patient group exceeded that of the control group 11 - to 24-fold. Incubation of patients’ LDE with adrenalin and obsidan revealed tolerance of adrenergic stimulation and blockade. The rosette-forming capacity of patients’ HDE did not differ spectacularly from the control group’s, though its HDE incubation with adrenalin resulted in a stronger suppression of rosette formation, while the adrenergic blockade considerably increased the number of rosette-forming eosinophils. Patients’ native serum demonstrated a capacity to strongly increase the quantity of rosette-forming HDE in healthy persons. This capacity was far greater pronounced in BA patients. BA and atopic dermatitic patients revealed a more pronounced abnormality of eosinophilic characteristics, while these numerical and qualitative characteristics in chronic opisthorchiatic patients were somewhere in between the sick and healthy groups.
About the Authors
A. B. Berestetzky
АОЗТ МТФ “Авиценна”;
МО “ Новая больница”
Russian Federation
Russian Federation
I. V. Leshenko
АОЗТ МТФ “Авиценна”;
МО “ Новая больница”
Russian Federation
Russian Federation
E. K. Beltukov
АОЗТ МТФ “Авиценна”;
МО “ Новая больница”
Russian Federation
Russian Federation
S. D. Popova
АОЗТ МТФ “Авиценна”;
МО “ Новая больница”
Russian Federation
Russian Federation
I. A. Kardashina
АОЗТ МТФ “Авиценна”;
МО “ Новая больница”
Russian Federation
Russian Federation
References
1. Анаев Э.Х. Эозинофилы и их морфологические и функциональные свойства при лечении бронхиальной астмы / / Пульмонология.— 1996.— № 3.— С.54—57.
2. Берестецкий А.Б. Опыт применения адекватных по эозинофильному тесту доз кортикостероидов в комплексном лечении больных бронхиальной астмой: Автореф. дис. ... канд. мед. наук.— Л., 1989.
3. Куделя Л.М. Бронхиальная астма и описторхоз — взаимоотягчающие заболевания / / Национальный конгресс по болезням органов дыхания, 6-й: Сборник резюме.— Новосибирск, 1996.— С.366.— № 1394.
4. Мармолевская Г.С., Берестецкий А.Б., Сергеева Л.Н. О роли эозинофилов при бронхиальной астме / / Тер.арх.— 1989.— №5.— С.91—94.
5. Попова С.Д. Анализ популяции эозинофилов и других лейкоцитов периферической крови при обострении атопического дерматита и хроническом описторхозе: Автореф. дис. ... канд. мед. наук.— Челябинск, 1994.
6. Baigelman W., Chodosh S., Pizzuto D., Cuples L.A. Sputum and blood eosinophils during corticosteroid treatment of acute exacerbations of asthma / / Am. J. Med.— 1983.— Vol.75, № 6.— P.929—936.
7. Capron M., Jonault T. IgE receptors on human eosinophils and eosinophil geterogenity / / Ann. Inst. Pasteur Immunol.— 1986.— Vol.137, N°. 3.— P.371—374.
8. Dahl. R., Venge P., Ollson I. Variations of blood eosinophils and eosinophil cationic protein in serum in patients with bronchial asthma: studies during inhalation challenge test / / Allergy.— 1978.— Vol.33, N° 4.— P.211—215.
9. Fukuda Т., Dunnette S.L., Reed C.E. et al. Increatsed number of hypodense eosinophils in the blood of patients with bronchial asthma / / Am. Rev. Respir. Dis.— 1985.— Vol. 132, № 5.— P.981—985.
10. Gleich G.J. The function of eosinophils / / Ann. Inst. Pasteur Immunol.— 1986.— Vol.l37D, № 1.— P .136—141.
11. Jondal М. Использование теста розеткообразования с эритроцитами барана в качестве маркера человеческих Т-лимфоцитов / / Лимфоциты: выделение, фракционирование и характеристика / Под ред. Дж.Натвига, П.Перлманна, Х.Вигзелля: Пер. с англ.— М.: Медицина, 1980.— С. 130.
12. Kaufman H.F., Vander Belt В., deMonchy J.G.R. et al. Leukotriene C4 production by normal density and low-density eosinophils of atopic individuals and other patients with eosinophilia / / J. Allergy Clin. Immunol.— 1987.— Vol.79, N° 4.— P.611—619.
13. Kay A.B. Eosinophils as effector cells in immunity and hypersensitivity disorders / / Clin. Exp. Immunol.— 1985.— Vol.62, № 1.— P.l — 12.
14. Parish W.E., Luckhurst E. Eosinophilia VI, spontaneous synthesis of chemokinetic, chemotactic, complement receptor-inducing activities of eosinophils by bronchial T lymphocytes of asthmaticbronchitic patients / / Clin. Allergy.— 1982.— Vol.12, N° 5 . 475—488.
15. Prin L., Capron M., Tonnel A .B. et al. Heterogenity of human peripheral blood eosinophils : variability in cell density and cytotoxic ability in relation to the level and the origin of hypereosinophilia / / Int. Arch. Allergy Appl. Immunol.— 1983.— Vol.72.— P.336—346.
16. Schatz M., Wasserman S., Patterson R. The eosinophil and the lung //A rch . Intern. Med.— 1982.— Vol. 142, N° 8.— P.1515—1519.
17. Sedgvick J.B., Frick W.E., Soudel P.M. et al. The appeareance of hypodense eosinophils during interleukin-2 treatment / / J. Allergy Clin. Immunol.— 1990.— Vol.85, N°. 3.— P.557—566.
18. Sorice F., De Simone С. Human eosinophil heterogenity / / Ric. Clin. Lab.— 1986.— Vol.16, N°. 3.— P.429—434.
19. Spry C.J.F. New properties and roles for eosinophils in disease : discussion paper / / J.R.Soc. Med.— 1985.— Vol.78, N° 10.— P.844—848.
20. The Eosinophil in Health and Disease / Ed. A.A.F.Mahmoud, K.F.Austen.— New York, 1980.— 364 p.
21. Weller P.F. Eosinophilia / / J. Allergy Clin. Immunol.— 1984.— Vol.73, N° 1.— Pt 1.— P.l — 10.
22. Wardlaw A.J., Kay A .B. The role of the eosinophil in the pathogenesis of asthma / / Allergy.— 1987.— Vol.42, N<> 5.— P.321—335.
23. Wiggins C., Patterson R. Hilar adenopathy, asthma and eosinophilia in a 17-year-old girl / / Ann. Allergy.— 1988.— Vol.60, N° 6.— P.497—498.
Review
For citations:
Berestetzky A.B., Leshenko I.V., Beltukov E.K., Popova S.D., Kardashina I.A. The differences of characteristics of blood eosinophils in patients affected with bronchial asthma, atopic dermatitis and opisthorchiasis. PULMONOLOGIYA. 1997;(4):34-42. (In Russ.)
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