Ciliary activity of ciliated epithelium cells in patients with lower airway infection (pneumonia and acute bronchitis)

Abstract

Ciliary activity of airway epithelium (CE) is investigated in different clinical and etiological forms of pneumonia and acute bronchitis. The study was carried out using a computed analyzer for biological objects’ mobility Cilia-03-08. The registration of ciliary mobility was performed by the method of transilluminate microscopy of mobile objects. Mathematical analysis of ciliar activity parameters was made by modulus programmes kit for ECM. Ciliary dysfunction is developed in patients with pneumonia and acute bronchitis and is accompanied by diminishing of ciliary beating frequency, change of phases duration ratio of ciliary motion cycle, decrease in synchronism of ciliary vibration in neighbouring epithelium areas. A correlation of ciliary motion parameters with kind of pathology, its etiology and course was found. The greatest inhibition of ciliary activity was observed in patients with pneumonia and acute bronchitis caused by Staphylococcus. Lingering pneumonia and acute bronchitis were accompanied by slower ciliary beating frequency. Ciliary dysfunction in pneumonia and acute bronchitis mainly due not to decrease in ciliary beating frequency but disorders of ciliary vibration synchronism in neighbouring epithelium areas. In recovery period the ciliary activity parameters do not return to normal independently pneumonia and acute bronchitis nosology. The mathematical method for ciliary motion analysis which has been described allows to evaluate quantitatively the ciliary function of respiratory epithelium and enables to detect its disorders in early stages using the criteria suggested.