Mechanisms of dexametazone-induced apoptosis of lymphocytes in atopic bronchial asthma
Abstract
Study results of spontaneous and dexamethazone (DEX)-induced or Ca2+ ionophor-induced apoptosis of peripheral blood lymphocytes (PBL) in healthy donors and atopic asthma (BA) patients are shown at the present work. The apoptosis was evaluated by several parameters: change in mitochondrial potential (MP), a ievel of phosphatidylserine (PS) expression on the outer leaflet of the cell membrane, forward (FSC) and side scatter (SSC) parameters and DNA fragmentation using flow cytometry. PBL of BA patients incubated in a culture showed later and less intensive DNA fragmentation in comparison with the controls. There were no differences between spontaneous MP and PS dynamics between the study groups. The DEX induced DNA fragmentation significantly earlier and greater after 72, 96 and 120 hrs of the incubation in the BA patients than in the controls. PBL incubation with DEX in the BA patients showed a significant decrease in the MP after 48 hrs of the incubation and later compared with the donors. The DEX-induced MP reduction correlated with the increase in the PS expression level. The analogous results were obtained for the Ca2+ ionophor-induced apoptosis. They demonstrated the resistance of PBL to spontaneous apoptosis in BA patients. The DEX-induced apoptosis of PBL was mitochondria-dependent. The PBL sensitivity to the DEX-induced apoptosis in the BA patients may be provided by their MP significant shifts compared with the controls.
About the Authors
S. V. Boichuk
Казанский государственный медицинский университет; Республиканский Центр по борьбе со СПИДом Минздрава РТ; Казанская государственная медицинская академия
Russian Federation
Russian Federation
I. S. Mustafin
Казанский государственный медицинский университет; Республиканский Центр по борьбе со СПИДом Минздрава РТ; Казанская государственная медицинская академия
Russian Federation
Russian Federation
R. S. Fassakhov
Казанский государственный медицинский университет; Республиканский Центр по борьбе со СПИДом Минздрава РТ; Казанская государственная медицинская академия
Russian Federation
Russian Federation
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Review
For citations:
Boichuk S.V., Mustafin I.S., Fassakhov R.S. Mechanisms of dexametazone-induced apoptosis of lymphocytes in atopic bronchial asthma. PULMONOLOGIYA. 2003;(2):10-16. (In Russ.)
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