Application of ELISA and immunoblotting methods for detection of anti-recoverin autoantibodies in sera of small-cell lung carcinoma patients
Abstract
Paraneoplastic (onconeural) antigens involve normal high-specific for the nervous system proteins which could be also expressed by tumor cells outside the nervous system. While entering the blood vessels paraneoplastic antigens initiate an autoimmune process resulted in production of autoantibodies which evoke paraneoplastic neurological syndrome occurrence. One of the paraneoplastic antigens — Ca2+-binding protein recoverin — can be expressed by some tumors' cells and induce autoimmune injury of the retina. We compared ELISA and immunoblotting methods to detect autoantibodies against recoverin in sera of lung cancer patients and demonstrated the ELISA to be non-specific whereas the immunoblotting is quite sensitive and specific for serial analysis of sera of lung cancer patients to detect the anti-recoverin autoantibodies.
About the Authors
A. V. Bazhin
НИИ физико-химической биологии им. А.Н.Белозерского МГУ;
Институт пульмонологии Минздрава РФ
Russian Federation
Russian Federation
O. N. Shifrina
НИИ физико-химической биологии им. А.Н.Белозерского МГУ;
Институт пульмонологии Минздрава РФ
Russian Federation
Russian Federation
M. S. Savchenko
НИИ физико-химической биологии им. А.Н.Белозерского МГУ;
Институт пульмонологии Минздрава РФ
Russian Federation
Russian Federation
N. K. Tikhomirova
НИИ физико-химической биологии им. А.Н.Белозерского МГУ;
Институт пульмонологии Минздрава РФ
Russian Federation
Russian Federation
A. G. Chuchalin
НИИ физико-химической биологии им. А.Н.Белозерского МГУ;
Институт пульмонологии Минздрава РФ
Russian Federation
Russian Federation
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Review
For citations:
Bazhin A.V., Shifrina O.N., Savchenko M.S., Tikhomirova N.K., Chuchalin A.G. Application of ELISA and immunoblotting methods for detection of anti-recoverin autoantibodies in sera of small-cell lung carcinoma patients. PULMONOLOGIYA. 2003;(3):62-65. (In Russ.)
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