Identification of drug resistance and genotyping of clinical isolates of Mycobacterium tuberculosis using the TB-TEST

Summary. Drug resistance and genotype of Mycobacterium tuberculosis were analyzed in 266 DNA samples isolated from clinical material of patients with TB using an experimental test system TB-TEST. This method is bases on oligonucleotide microarray technology and allows determination most prevalent in Russia genotypes of M. tuberculosis and genomic mutations responsible for drug resistance to the main medications used for treatment of TB: rifampicin, isoniazid, ethambutol, fluoroquinolones, aminoglycosides, and capreomycin. As a result, drug resistance profiles and genotypes were identified for all samples. The test results were highly related to results of conventional microbiological tests. The TB-TEST could be used as a preferred laboratory method for diagnosis of TB.

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