An ultrastructural observation of lungs treated by the hypoxic therapy: an assessment of its therapeutic efficacy as one of the impulse therapy
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Abstract
Intermittent hypoxic training (IHT) has been used for prevention and treatment of some diseases and for training athletes. In this study, we examined the morphological changes in lungs after IHT using light and electron microscopy. 39 male Wistar rats were sustained intermittent hypoxic training (10 % 0 2) for 5 min three times a day with 5-m in rebreathing intervals or 15, 30, 60 min once a day for 21 consecutive days, respectively. Results showed that pulmonary dam age occurred at 3-rd day of IHT, but one week later the pulmonary dam age repaired in IHT animals with brief-time hypoxia (5 or 15 min), but 30-m in and 60-m in IHT groups showed pulmonary dam age until 21- st day. Ultrastructurely, lungs in 5-m in and 15-m in IHT groups were characterized by hyperplasia of alveolar type II cells, which may enable them more rapid repairing of hypoxic lesions and may represent a key mechanism of the IHT. Those findings dem onstrate that IHT could be justified as the impulse therapy for the cells and tissues.
About the Authors
Kodo OkuyamaJapan
Jingtao Jiang
Japan
Kaoru Aihara
Japan
References
1. Strelkov R.B. On the possible clinical use of a gas hypoxic mixture of the radioprotectant? In: Annual meeting of the European society for radiation biology: Book of abstracts. Rome; 1972. 406.
2. Serebrovskaya T., Swanson R., Karaban I.N. et al. Intermittent hypoxia alters hypoxic ventilatory responses. Fiziol. Zh. 1999; 45 (5): 9-18.
3. Serebrovskaya T., Swanson R., MankovskayaI. et al. Training with intermittent hypoxia influence free radical processes and glutathione antioxidant system enzymes in bronchial asthma patients. Allerg. Immunol. 1995; 27: 266.
4. Howard L.S., Robbins P.A. Alterations in respiratory control during 8 h of isocapnic and poikilocapnic hypoxia in humans. J. Appl. Physiol. 1995; 78 (3): 1098-1107.
5. Adiiatulin A.L, Piliavskaia A.N., Takch.uk E.N, Guliaeva N.V. Various mechanism s of protective action of interval hypoxic training during preparation for abdominal delivery. Patol. Fiziol. Eksp. Ter. 1997; 3: 26-29.
6. Cao K.Y, Zwilhch C.W, Berthon-Jones M., Sullivan C.E. Increased normoxic ventilation induced by repetitive hypoxia in conscious dogs. J. Appl. Physiol. 1992; 73 (5): 2083-2088.
7. Vizek M., Picklett C.K., Weil J.V. Biphasic ventilatory response of adult cats to sustained hypoxia has central origin. J. Appl. Physiol. 1987; 63 (4): 1658-1664.
8. Borel C.O., Guy J., Barcik U. et al. Effect of hypobaria on ventilatory and C 0 2 responses to short-term hypoxic exposure in cats. Respir. Physiol. 1998; 101 (1): 45 - 53.
9. Abramov A.V. Effect of the intermittent hypoxic training on the functioning of peptidergic neurons of the paraventricular hypothalamic nucleus and brain stem neurons in rats. Ross. Fiziol. Zh. im I. M. Sechenova 1998; 84 (3): 1 73-181.
10. Olano M., Song D., Murphy S. et al. Relationships of dopamine, cortical oxygen pressure, and hydroxyl radicals in brain of newborn piglets during hypoxia and posthypoxic recovery. J. Neurochem. 1995; 65 (3): 1205-1212.
11. Solo-Arape I., Burton M.D., Kazemi H. Central amino acid neurotransmitters and the hypoxic ventilatory response. Am. J. Respir. Crit. Care Med. 1995; 151 (4): 1113-1G20.
12. Mason R.J. Role of alveolar type II epithelial cells in interstitial lung disease. J. Jpn. Med. Soc. Bio. Interface 1988; 19: 1 -9.
13. Goerke J. Lung surfactant. Biochim. Biophys. Acta 1974; 344: 244-305.
14. Sywabe A., Takahashi K., Saito Y. et al. Surfactant secretion from alveolar type II cells. Jpn. Med. Soc. Bio. Interface 1995; 26: 37-47.
15. Ota/& K., Suwabe A., Yakuwa N. et al. Effects of artificial surfactant (surfacten) on human neutrophic functions. J. Jpn. Med. Soc. Bio. Interface 1993; 24: 84 -92 .
16. Wilsher M.L. Immunoregulatory properties of pulmonary surfactant: effect of lung lining fluid on proliferation of hum an blood lymphocytes. Thorax 1988; 43: 354-359.
17. Van Iwaarden E.B., Wehners J., Verhoej H.P. et al. Pulmonary surfactant protein A enhances the host-defense mechanism of rat alveolar macrophages. Am. J. Respir. Cell Mol. Biol. 1990; 2: 9118.
Review
For citations:
Okuyama K., Jiang J., Aihara K. An ultrastructural observation of lungs treated by the hypoxic therapy: an assessment of its therapeutic efficacy as one of the impulse therapy. PULMONOLOGIYA. 2004;(4):67-71. (In Russ.)
ISSN 2541-9617 (Online)