Genetic homeostasis disorders in bronchial asthma children

Abstract

Genetic lesions of somatic cells (lymphocytes, erythrocytes, leukocytes, cheek epithelium cells) and biollquids (blood plasma) in patients with various severity of atopic bronchial asthma and healthy children were studied using methods of chromosom e aberration account in blood lymphocytes, analysis of micronuclei in blood erythrocytes and epithelial cells of the cheek internal surface, sedimentation velocity analysis of the leukocyte DNA integrity, chromosom e aberration account in seeds of Crepis ca pillaris. Levels of lymphocyte chromosom e aberrations and m icronuclei in the erythrocytes and the cheek epithelium cells were higher in the asthmatic children than in healthy ones. Amount of the leukocyte DNA breaks in bronchial asthma children was also more than in healthy.

Experiments with the blood plasma of the asthmatic and non-asthmatic children revealed a tendency to increase a clastogenic activity as the plasma concentrations grew (50 and 100%). The plasma clastogenic activity in 25% of both the groups was not found. So, the metabolic disorders in asthmatic children were accompanied by occurrence of clastogenic factors in the cells and intercellular environment, decreasing in influence of antimutagenic systems and in elimination of aberrant cells.