Fas (CD95) antigen and Tumor necrosis factor-α blood concentration in patients with different manifestations of tuberculosis
https://doi.org/10.18093/0869-0189-2015-25-4-456-460
Abstract
The aim of this study was to investigate Fas (CD95) and Tumor necrosis factor
α (TNF α) expressions in patients with different manifestations of tuberculosis (TB).
Methods. The study involved 54 patients with active pulmonary TB of different severity and 22 healthy volunteers. CD95 cell number was counted by lymphocyte immunophenotyping. Spontaneous and stimulated TNF α production by Mycobacterium bovis (BCG) was assessed in the supernatant of 24h blood mononuclear cell culture using the ELISE method.
Results. Patients with severe and complicated TB had significantly increased both CD95 number and spontaneous TNF α production in comparison to patients with milder TB course and to healthy volunteers. CD95 cell number was associated with cavitation and positive culturing of Mycobacterium tuberculosis.
Conclusion. These results improve our knowledge of immunopathology of TB and facilitate further search for targeted immune rehabilitation.
About the Authors
T. Yu. Salina
State Institution "V.I.Razumovskiy Saratov State Medical University", Healthcare Ministry of Russia: 112, Bol. Kazach'ya str., Saratov, 410012, Russia
Russian Federation
Russian Federation
MD, Associate Professor at Department of Phthisiatics, Physician Postgraduate Education Faculty, State Institution "V.I.Razumovskiy Saratov State Medical University", Healthcare Ministry of Russia; tel.: (8452) 265608
T. I. Morozova
State Institution "V.I.Razumovskiy Saratov State Medical University", Healthcare Ministry of Russia: 112, Bol. Kazach'ya str., Saratov, 410012, Russia
State Institution "Regional Cliniical Tuberculosis Institution": 22, Vol'skaya, Saratov, 410056, Russia
Russian Federation
Russian Federation
MD, Professor, Head of Department of Phthisiatics, Physician Postgraduate Education Faculty, State Institution "V.I.Razumovskiy Saratov State Medical University", Healthcare Ministry of Russia; Hospital Chief Executive Officer at State Institution "Regional Cliniical Tuberculosis Institution"; tel. / fax: (8452) 261690
References
1. Yarilin A.A. Immunology. Moscow: GEOTAR Media; 2010 (in Russian).
2. Borzakova S.N., Aksenova V.A., Reyzis A.R. Lymphocyte apoptosis in antituberculosis druginduced hepatic injury. Gastroenterologiya. 2012; 9 (77): 67–72 (in Russian).
3. Shil'ko T.A. Pathogenic factors modulating blood mononuclear cell apoptosis in pulmonary tuberculosis: Dis. Tomsk; 2009 (in Russian).
4. Roitt I., Brostoff D.J., Male D. Immunology. Translated from English. Moscow: Mir; 2000 (in Russian).
5. Dheda K., Schwander S.K., Zhu B. et al. The immunology of tuberculosis: From bench to bedside. Respirology. 2010; 15 (3): 433–450.
6. Dunaev P.D., Boychuk S.V., Mustafin I.G. Properties and a role of TNF α for the pathogenesis of HIV infection. Kazanskiy meditsinskiy zhurnal. 2012; 93 (2): 290–293 (in Russian).
7. Esimova I.E., Urazova O.I., Novitskiy V.V. et al. Causes of immune response dysregulation in pulmonary tuberculosis: a role of M. tuberculosis. Byulleten' sibirskoy meditsiny. 2012; 3: 79–86 (in Russian).
8. Mordyk A.V., Tsygankova E.A., Puzyreva L.V., Turitsa A.A. Anti tuberculosis immunity and mechanisms of its development (review). Dal'nevostochnyy meditsinskiy zhurnal. 2014; 1: 126–130 (in Russian).
9. Voronkova O.V., Urazova O.I., Novitskiy V.V. et al. Immuneimbalance in different clinical and pathogenic variants of acute progressive pulmonary tuberculosis. Byulleten' sibirskoy meditsiny. 2010; 3: 42–50 (in Russian).
10. Novikov P.D., Novikov D.K. A comparison of methods of lymphocyte immunophenotyping. Immunopatologiya, aller gologiya, infektologiya. 2000; 1: 62–66 (in Russian).
Review
For citations:
Salina T.Yu., Morozova T.I. Fas (CD95) antigen and Tumor necrosis factor-α blood concentration in patients with different manifestations of tuberculosis. PULMONOLOGIYA. 2015;25(4):456-460. (In Russ.) https://doi.org/10.18093/0869-0189-2015-25-4-456-460
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