Mixed bacterial-viral lung infections in patients with cystic fibrosis
E. M. Burmistrov,
K. G. Krasnoslobodtsev,
L. R. Avetisyan,
E. A. Siyanova,
O. S. Medvedeva,
E. G. Tselikina,
N. B. Polyakov,
A. I. Solovyev,
A. Yu. Voronkova,
E. I. Kondratyeva,
S. A. Krasovskiy,
V. G. Zhukhovitsky,
E. I. Burtseva,
N. A. Nikitenko,
M. Yu. Chernukha https://doi.org/10.18093/0869-0189-2023-33-4-488-496
Abstract
The main cause of death in patients with cystic fibrosis (CF) is infectious process in the lungs, in particular, chronic lung infections caused by various pathogens, most often a combination of bacteria, fungi, or viruses. Data on mixed bacterial and viral-bacterial infections from domestic and foreign sources are fragmentary and sparse. The dominant associations of bacterial and viral pathogens in patients with cystic fibrosis have not been studied properly, and data on their epidemiological significance are lacking. The aim of this study was to assess the prevalence of bacterial and viral infections in patients with cystic fibrosis and to substantiate the need for the development of virological monitoring. Methods. Biomaterials from the respiratory tract of CF patients (409 children and 160 adults with CF) examined from 2006 to 2022 were used. The study was carried out using bacteriological methods, molecular genetic methods (RT-PCR) and MALDI-TOF mass-spectrometry. Results. Microflora of the respiratory tract was shown to be mixed in 2/3 patients with CF. The microflora of the lungs of children with CF is a dynamic community of microorganisms with high diversity and variability. In adult patients, associations of microorganisms are more common than in children, but the composition of associations is less diverse. We isolated about 40 species of bacteria from adult patients and more than 85 species from children in our sample. NFMO prevailed, including Burkholderia cepacia complex, Pseudomonas aeruginosa, Achromobacter xylosoxidans, Achromobacter ruhlandii, Stenotrophomonas maltophilia, and Staphylococcus aureus, Candida albicans, Aspergillus spp. Real-time PCR showed the presence of rhinovirus RNA in 10% of samples obtained from children and 12.9% from adults with cystic fibrosis. Conclusion. Our results indicate the need for continuous monitoring of the lung microflora in patients with CF, including testing for viruses.
Supporting agencies: The article was posted with the support of FORMED.
About the Authors
E. M. Burmistrov
Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”
Russian Federation
Competing Interests:
Russian Federation
Egor M. Burmistrov - Researcher, Laboratory of Molecular Epidemiology of Nosocomial Infections, Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”.
Gamaleya ul. 18, Moscow, 123098
tel.: (499) 193-55-94
Competing Interests:
None
K. G. Krasnoslobodtsev
Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”
Russian Federation
Competing Interests:
Russian Federation
Kirill G. Krasnoslobodtsev - Researcher, Laboratory of Etiology and Epidemiology of Influenza, Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”.
Gamaleya ul. 18, Moscow, 123098
tel.: (499) 193-55-94
Competing Interests:
None
L. R. Avetisyan
Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”
Russian Federation
Competing Interests:
Russian Federation
Lusine R. Avetisyan - Doctor of Medicine, Leading Researcher, Laboratory of Epidemiology of Nosocomial Infections, Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”.
Gamaleya ul. 18, Moscow, 123098
tel.: (499) 193-55-94
Competing Interests:
None
E. A. Siyanova
Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”
Russian Federation
Competing Interests:
Russian Federation
Ekaterina A. Siyanova - Junior Researcher, Laboratory of Epidemiology of Nosocomial Infections, Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”.
Gamaleya ul. 18, Moscow, 123098
tel.: (499) 193-55-94
Competing Interests:
None
O. S. Medvedeva
Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”
Russian Federation
Competing Interests:
Russian Federation
Olga S. Medvedeva - Junior Researcher, Laboratory of Epidemiology of Nosocomial Infections, Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”.
Gamaleya ul. 18, Moscow, 123098
tel.: (499) 193-55-94
Competing Interests:
None
E. G. Tselikina
Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”
Russian Federation
Competing Interests:
Russian Federation
Eugenia G. Tselikina - Junior Researcher, Laboratory of Epidemiology of Nosocomial Infections,, Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”.
Gamaleya ul. 18, Moscow, 123098
tel.: (499) 193-55-94
Competing Interests:
None
N. B. Polyakov
Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”
Russian Federation
Competing Interests:
Russian Federation
Nikita B. Polyakov - Researcher, Laboratory of Indication and Ultrastructur-al Analysis of Microorganisms, Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”.
Gamaleya ul. 18, Moscow, 123098
tel.: (499) 190-74-22
Competing Interests:
None
A. I. Solovyev
Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”
Russian Federation
Competing Interests:
Russian Federation
Andrey I. Solovyev - Doctor of Biology, Researcher, Laboratory of Indication and Ultrastructural Analysis of Microorganisms, Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F.Gamaleya of the Ministry of Health of the Russian Federation”.
Gamaleya ul. 18, Moscow, 123098
tel.: (499) 190-74-22
Competing Interests:
None
A. Yu. Voronkova
Federal State Budgetary Scientific Institution “Research Centre for Medical Genetics”, Ministry of Science and Higher Education of the Russian Federation; Moscow Region State Budgetary Healthcare Institution “Research Clinical Institute of Childhood, Ministry of Health of the Moscow Region”
Russian Federation
Competing Interests:
Russian Federation
Anna Yu. Voronkova - Candidate of Medicine, Leading Researcher, Scientific and Clinical Department of Cystic Fibrosis, Federal State Budgetary Scientific Institution “Research Centre for Medical Genetics”, Ministry of Science and Higher Education of the Russian Federation; Pediatrician, Department of Cystic Fibrosis, Moscow Region State Budgetary Healthcare Institution “Research Clinical Institute of Childhood, Ministry of Health of the Moscow Region”.
Moskvorechye ul. 1, Moscow, 115522; Bolshaya Serpukhovskaya ul. 62, Moscow, 115093
tel.: (495) 111-03-03
Competing Interests:
None
E. I. Kondratyeva
Federal State Budgetary Scientific Institution “Research Centre for Medical Genetics”, Ministry of Science and Higher Education of the Russian Federation; Moscow Region State Budgetary Healthcare Institution “Research Clinical Institute of Childhood, Ministry of Health of the Moscow Region”
Russian Federation
Competing Interests:
Russian Federation
Elena I. Kondratyeva - Doctor of Medicine, Professor, Head of the Scientific and Clinical Department of cystic fibrosis, Federal State Budgetary Scientific Institution “Research Centre for Medical Genetics”, Ministry of Science and Higher Education of the Russian Federation; Deputy Director for Science, Moscow Region State Budgetary Healthcare Institution “Research Clinical Institute of Childhood, Ministry of Health of the Moscow Region”.
Moskvorechye ul. 1, Moscow, 115522; Bolshaya Serpukhovskaya ul. 62, Moscow, 115093
tel.: (495) 111-03-03
Competing Interests:
None
S. A. Krasovskiy
Federal State Budgetary Institution “Pulmonology Scientific Research Institute” under Federal Medical and Biological Agency of Russian Federation
Russian Federation
Competing Interests:
Russian Federation
Stanislav А. Krasovskiy - Candidate of Medicine, Senior Researcher, Cystic Fibrosis Laboratory, Federal State Budgetary Institution “Pulmonology Scientific Research Institute” under Federal Medical and Biological Agency of Russian Federation; Leading Researcher.
Orekhovyy bul’var 28, Moscow, 115682
tel.: (495) 965-23-24
Author ID: 688178
Competing Interests:
None
V. G. Zhukhovitsky
Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”; Federal State Budgetary Educational Institution of Additional Professional Education “Russian Medical Academy of Continuous Professional Education”, Ministry of Health of Russia
Russian Federation
Competing Interests:
Russian Federation
Vladimir G. Zhukhovitsky - Candidate of Medicine, Head of the Laboratory of Indication and Ultrastructural Analysis of Microorganisms, Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”; Associate Professor, Department of Biochemistry and Immunopathology, Federal State Budgetary Educational Institution of Additional Professional Education “Russian Medical Academy of Continuous Professional Education”, Ministry of Health of Russia.
Gamaleya ul. 18, Moscow, 123098; Barrikadnaya ul. 2/1, Moscow, 123995
tel.: (499) 193-43-76
Competing Interests:
None
E. I. Burtseva
Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”
Russian Federation
Competing Interests:
Russian Federation
Elena I. Burtseva - Doctor of Medicine, Head of Laboratory of Etiology and Epidemiology of Influenza, Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”.
Gamaleya ul. 18, Moscow, 123098
tel.: (499) 193-55-94
Competing Interests:
None
N. A. Nikitenko
Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”
Russian Federation
Competing Interests:
Russian Federation
Natalia A. Nikitenko - Candidate of Biology, Senior Researcher at Cell Microbiology Laboratory, Head of the Medical Department, Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”.
Gamaleya ul.18, Moscow, 123098
tel.: (499) 193-55-94
Competing Interests:
None
M. Yu. Chernukha
Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya of the Ministry of Health of the Russian Federation”
Russian Federation
Competing Interests:
Russian Federation
Marina Yu. Chernukha - Doctor of Medicine, Head of the Laboratory of Epidemiology of Nosocomial Infections, Federal Government Budgetary Institution “National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F.Gamaleya of the Ministry of Health of the Russian Federation”.
Gamaleya ul. 18, Moscow, 123098
tel.: (499) 193-61-17
Competing Interests:
None
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Burmistrov E.M., Krasnoslobodtsev K.G., Avetisyan L.R., Siyanova E.A., Medvedeva O.S., Tselikina E.G., Polyakov N.B., Solovyev A.I., Voronkova A.Yu., Kondratyeva E.I., Krasovskiy S.A., Zhukhovitsky V.G., Burtseva E.I., Nikitenko N.A., Chernukha M.Yu. Mixed bacterial-viral lung infections in patients with cystic fibrosis. PULMONOLOGIYA. 2023;33(4):488-496. (In Russ.) https://doi.org/10.18093/0869-0189-2023-33-4-488-496
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