Influence of trombocytes on generation of active oxygen forms with blood leukocytes, peroxide lipid oxidation, and antiperoxide activity in patients with bronchial asthma
Abstract
56 patients with bronchial asthma (BA) were examined to evaluate features of the influence of trombocytes on active form oxygen generation (AFOG), peroxide oxidation of lipids (POL), and the antiperoxide activity of blood serum. AFOG and the serum peroxide activity were determined with the luminol dependent hemiluminescent (HL) method. The effect of trombocytes on AFOG was evaluated with comparison of delta-hemiluminescence (HL) under the influence o f serum with the trombocytes poverty (STP) and the one with the trombocytes excess (STE). The effect of trombocytes on HL of serum was determined with comparison of delta-HL of STP and STE under the H2O2 influence. The effect of trombocytes on POL was evaluated with comparison of the malon aldehyd content in STP and in STE. In comparison with healthy donors there was the decrease o f the inhibiting influence of trombocytes on AFOG (at 1.6 times), POL (at 1.2 times), and serum HL (at 1.5 times) in patients with the exacerbation phase of the disease. During the remission phase, the trombocytes inhibiting influence on AFOG, POL, and serum HL increased. The direct proportion was found between the decrease of the inhibited influence of trombocytes on AFOG, POL and serum HL and the severity of exacerbation. The great decrease of the inhibited influence of trombocytes on AFOG, POL, and serum HL was found in patients with steroid dependent and aspirin-induced asthma in comparison with those with nonsteriod dependent and atopic asthma. Thus, the obtained data testify about the inclusion of trombocytes into the pathogenesis of BA by means of the decrease of their function of inhibition of free radical processes.
About the Authors
I. G. Daniliack
ММА им. И.М. Сеченова
Russian Federation
Russian Federation
A. H. Kogan
ММА им. И.М. Сеченова
Russian Federation
Russian Federation
S. Bolevic
ММА им. И.М. Сеченова
Russian Federation
Russian Federation
A. A. Stremoukhov
ММА им. И.М. Сеченова
Russian Federation
Russian Federation
Sa. Bolevic
ММА им. И.М. Сеченова
Russian Federation
Russian Federation
References
1. Аматуни В.Г., Егоян А.К., Нариманов М.З. и др. Тучноклеточный механизм патогенеза бронхиальной астмы и перекисное окисление мембранных липидов / / Тер. арх.— 1989.— No 3.— С.34—37.
2. Болевич С. Свободнорадикальные кислородные и липидные процессы и возможность их коррекции у больных бронхиальной астмой: Дис. ... канд. мед. наук.— М., 1991.
3. Величковская Е.Б. Диагностическое значение исследований хемилюминесценции моноцитов при атопической бронхиальной астме: Автореф. дис. ... канд. мед. наук.— М., 1986.
4. Владимиров Ю.А., Шерстнев М.П. Хемилюминесценции клеток животных.— М., 1989.
5. Даниляк И.Г., Коган А.Х., Болевич С. Генерация активных форм кислорода лейкоцитами у больных бронхиальной астмой / / Пульмонология.— 1991.— No 1.— С.39—42.
6. Коган А.Х., Медных А.Я., Николаев С.М. Резистентность иммунизированных тканей к перекиси водорода свободнорадикального окисления / / Свободнорадикальное окисление липидов в норме и патологии.— М., 1976.— С.76—78.
7. Коган А.Х., Лосев Н.И., Цыпин А.Б. и др. Генерация активных микробицидных форм кислорода лейкоцитами через сосудистое русло легких / / Бюл. экспер. биол.— 1989.— No 6.— С.688—690.
8. А.с. 1735784 СССР. Способ определения супероксидного анион радикала при фагоцитозе / Коган А.Х., Ершов В.И., Алекперова Г.Р., Хапугина И.В., Перминов А.Н. / / Открытия.— 1992.— No 19.— С.46.
9. Федосеев Г.Б., Хлопотова Г.П. Бронхиальная астма.— Л., 1988.
10. Чучалин А.Г. Бронхиальная астма.— М., 1985.
11. Ameisen J.C., Joseph М., Tonnel А.В. et al. A role for platelets in allergy and asthma / / PAF in Asthma.— New York: Academic Press, 1986.— P.169—181.
12. Douset J.C., Trouith H., Foglieri M.J. Plasma malonaldehyde levels during myocardial infarction / / Clin. Chim. Acta.— 1983.— Vol.129.— P.319—322.
13. Geswani S.K., Ohashi М., Stathas P. et al. Platelet-activating factor stimulates secretion of respiratory glycoconjugate from human airways in culture / / J. Allergy Clin. Immunol.— 1989.— Vol.84.— P.726—734.
14. Heffner J.E., Cook J.A., Holuskka P.V. Human platelets modulate edema formation in isolated rabbit lungs / / J. Clin. Invest.— 1989.— Vol.84.— P.757—764.
15. Kay A.B. Leucocytes in asthma / / Immunol. Invest.— 1988.— Vol.17.— P.679—705.
16. Kurosawa М., Kobayashi H., Kobayashi S., Nakano M. Failure of inhibitory effects by platelets on super-oxide anion generation from stimulated neutrophils in a severe bronchial asthmatic / / Allergy.— 1991.— Vol.46.— P .173—179.
17. Meltzer S., Golberg B., Lad P., Easton J. Superoxide generation and its modulation by adenosine in the neutrophilis of subject with asthma / / J. Allergy Clin. Immunol.— 1989.— Vol.83.— P .960— 966.
18. Mannaioni P.F., Palmerani B., Pistelly A. et al. Histamine release by platelet aggregation / / A gents and A ctions.— 1990.— Vol.30.— P.44—48.
19. Sanjar S., Morley J., Smith D. Properties of platelet-activating factor (PAF) relevant to asthma / / PAF in Asthma.— New York: Academic Press, 1986.— P.59—75.
20. Sustiel A.M., Joseph B., Rocklin R.E., Borish L. Asthmatic patients have neutrophils that exhibit diminished responsivenese to adenosine / / Am. Rev. Respir. Dis.— 1989.— Vol. 140.— P .1556—1561.
Review
For citations:
Daniliack I.G., Kogan A.H., Bolevic S., Stremoukhov A.A., Bolevic S. Influence of trombocytes on generation of active oxygen forms with blood leukocytes, peroxide lipid oxidation, and antiperoxide activity in patients with bronchial asthma. PULMONOLOGIYA. 1994;(2):43-47. (In Russ.)
Views:
132
Email this article 