Polymerase chain reaction as a fast and high sensible method to discover the tuberculosis agent in biological materials
Abstract
The efficacy of polymerase chain reaction (PCR) for M.tuberculosis detection in biological materials was studied. Sputum and biological fluids were investigated in 106 patients with tuberculosis and non-specific pulmonary diseases. The observed specific and great PC R sensitivity was two times greater than the sensitivity of bacteriological culture in solid media.
A kit of reagents for rapid M.tuberculosis detection by PCR-technique has been developed.
It was shown that the improving and simplifying possibility of PCR technology by magnetic immunosorbent use allows to perform PC R without DNA isolation and keep the method sensitivity.
About the Authors
A. A. Priymack
Московский НИИ туберкулеза Минздрава РФ, ВНИИ сельхозбиотехнологии РАСХН
Russian Federation
Russian Federation
M. A. Vladimirskiy
Московский НИИ туберкулеза Минздрава РФ, ВНИИ сельхозбиотехнологии РАСХН
Russian Federation
Russian Federation
L. K. Shipinci
Московский НИИ туберкулеза Минздрава РФ, ВНИИ сельхозбиотехнологии РАСХН
Russian Federation
Russian Federation
A. N. Kaliuck
Московский НИИ туберкулеза Минздрава РФ, ВНИИ сельхозбиотехнологии РАСХН
Russian Federation
Russian Federation
T. S. Denisova
Московский НИИ туберкулеза Минздрава РФ, ВНИИ сельхозбиотехнологии РАСХН
Russian Federation
Russian Federation
B. S. Naroditskiy
Московский НИИ туберкулеза Минздрава РФ, ВНИИ сельхозбиотехнологии РАСХН
Russian Federation
Russian Federation
M. V. Kalinina
Московский НИИ туберкулеза Минздрава РФ, ВНИИ сельхозбиотехнологии РАСХН
Russian Federation
Russian Federation
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Review
For citations:
Priymack A.A., Vladimirskiy M.A., Shipinci L.K., Kaliuck A.N., Denisova T.S., Naroditskiy B.S., Kalinina M.V. Polymerase chain reaction as a fast and high sensible method to discover the tuberculosis agent in biological materials. PULMONOLOGIYA. 1995;(3):61-64. (In Russ.)
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