The biochemical methods of investigation of lung surfactant system
Abstract
Complex of modem biochemical approaches in study of lung surfactant content, composition and cell metabolism is analysed in the article. On the basis of received experimental results the advisability of investigation of the condensate of respiratory air as a substance, conteined lung surfactant, is called in question.
References
1. Кейтс М. Техника липидологии: Выделение, анализ и идентификация липидов: Пер. с англ. — М.: Мир, 1975.
2. Петрунина М.П. Клиническое изучение фиброза легких при туберкулезе // Пробл. туб. — 1973. — № 9. — С. ЗО—34.
3. Процюк Р.Г. // Сурфактантная и антисурфактантная система легких. — Харьков, 1990. — С. 66—68.
4. Сидоренко Г.И., Зборовский Э.И., Левина Д.И. Атравматический метод исследования поверхностно-активных свойств легкого (сурфактанта): Метод, рекомендации. — Минск, 1981.
5. Филипенко Л.И. Участие легочных макрофагов в регуляции количества сурфактанта на поверхности альвеол // Бюл. экспер. биол. — 1977. — № 12. — С. 646—650.
6. Шеллард Э. Количественная хроматография на бумаге и в тонком слое: Пер. с англ. — М., Мир, 1971.
7. Шталь Е. Хроматография в тонких слоях: Пер. с нем. — М.: Мир, 1965.
8. Яковлева О.А., Кучук А.П., Баяк Н.Ю., Зубко Е.В. Значение конденсата выдыхаемого воздуха в оценке влаговыделительной функции легких // Врач. дело. — 1987. — № 10. — С. 91—92.
9. Яковлева О.А. Диагностические возможности изучения конденсата выдыхаемого воздуха // Тер. арх. — 1990. — N° 3. — С. 102—107.
10. Barkai G., Mashiach S., Goldman В. et al. The effect of pressure on the lipid microviscosity and phase transition of lung surfactant // Biochim. Biophys. Acta. — 1985. — Voi. 834, N° 1. — P. 103—109.
11. Bernhard W., von der Hardt H. Bronchial surfactant: phospholipid classes and phosphatidylcholine molecular species as indicators of its alveolar origin // Appl. Cardipulmon. Pathophysiol. — 1995. — Vol. 5, Suppl. 3. — P. 6—7.
12. Blank M.L., Schmil J.A., Privett O.S. Quantitative analysis of lipids by thin-layer chromatography // J. Amer. Oil. Chem. Soc. — 1964.— Vol. 41, N° 5. — P. 371—376.
13. Cernansky G., Liau D.-F., Hashim S.A., Ryan S.F. Estimation of phosphatidylglycerol in fluids containing pulmonary surfactant // J. Lipid Res. — 1980. — Vol. 21, № 8. — P. l128—1131.
14. Chander A., Reicherter J., Fisher A.B. Degradation of dipalmitoylphosphatidylchoiline by isolated rat granular pneumocytes and reutilization for surfactant synthesis // J. Clin. Invest. — 1987. — Vol. 79, N° 4. — P. 1133—1138.
15. Claypool W.D., Wang D.L., Chander A., Fisher A.B. “Hydrophobic” surfactant-apoproteins and augmentation of phospholipid recycling // Exp. Lung. Res. — 1984. — Vol. 6, № 3—4. — P. 215—222.
16. Dobbs L.G., Mason R.J., Williams M.C. et al. Secretion of surfactant y primary cultures of alveolar type II cells isolated from rats // Biochim. Biophys. Acta. — 1982. — Vol. 713, № 1. — P. l18—127.
17. Downing D.T. Photodensitometry in the thin-layer chromatographic analysis of neutral lipids // J. Chromatogr. — 1968. — Vol. 38, N° 4. — Р. 91—99.
18. Dulley J., Grieve P. A simple technique for elimination interference by detergents in the Lowry method of protein determination // Anal. Biochem. — 1975. — Vol. 64, N° 1. — P. 136-141.
19. Eckert H., Lux Al., Lachman B. The role of alveolar macrophages in surfactant turnover // Lung. — 1983. — Vol. 161, N° 2. — P. 213—218.
20. Elledge B.W., Whitsett J.A. Structure of hydrophobic surfactant protein SP-B indicated by use of intrinsic tryptophan fluorescence // Am. Rev. Respir. Dis. — 1988. — Vol. 137, N° 4. — Suppl. — P. A285.
21. Folch J., Lees Al., Sloane-Stanley G.H. A simple method for the isolation and purification of total lipids from animal tissues // J. Biol. Chem. — 1957. — Vol. 226, N° 1. — P. 497—509.
22. Franson R., Beckerdite S., Wang P. et al. Some properties of phospholipases of alveolar macrophages // Biochim. Biophys. Acta. — 1973.— Vol. 296, N° 2. — P. 365—373.
23. Johannson J., Curstedt T., Robertson B. The proteins of the surfactant system // Eur. Respir. J. — 1994. — Vol. 7. — P. 372—391.
24. Kaplan R.S., Pedersen P.L. Determination of microgram quantities of protein the presence of milligram levels of lipid with amido black 10B // Anal. Biochem. — 1985. — Vol. 150, N° 1. — P. 97—104.
25. Khan A.Q., Sikpi Al.O., Das S.K. Phospholipid composition of guinea pig lung lavage // Lipids. — 1985. — Vol. 20, N° 1. — P. 7—10.
26. King R.J. Composition and metabolism of the apolipoproteins of pulmonary surfactant // Ann. Rev. Physiol. — 1985. — Vol. 47. — P. 775—788.
27. Kuroki Y., Mason R.J., Voelker D.R. Pulmonary surfactant apoprotein A. Structure and modulation of surfactant secretion by rat alveolar type II cells // J. Biol. Chem. — 1988. — Vol. 263, N° 7. — P. 3388—3394.
28. Lesur O., Bouhadiba T., Melloni B. et al. Alteration of surfactant lipid turnover in silicosis: evidence of a role for surfactant-associated protein A // Int. J. Exp. Pathol. — 1995. — Vol.76. — P. 287—298.
29. Lewis R.W., Harwood J.L., Richards R.J. A method for preparing radiolabeled rat pulmonary surfactant // Biochem. J. — 1986. — Vol. 235, N° 1. — P. 75—79.
30. Lowry O.H., Rosebrough N.J., Farr A.L. et al. Protein measurement with the pholin-phenol reagent // J. Biol. Chem. — 1951. — Vol. 193, N° 1. — P. 265—275.
31. Mason R.J., Nellenbogen J., Clements J.A. Isolation of disaturated phosphatidylcholine with osmium tetroxide // J. Lipid Res. — 1976. — Vol. 17, N° 3. — P. 281—284.
32. Mason R.J., Nellenbogen J. Synthesis of saturated phosphatidylcholine and phosphatidylglycerol by freshly isolated rat alveolar type II cells // Biochim. Biophys. Acta. — 1984. — Vol. 794, N° 3. — P. 392—402.
33. McMurchie E.J., Teubner J.K., Gibson R.A. Thermal phase transitions in sheep, rat and rabbit surfactant lipids detected by differential scanning colorimetry // Comp. Biochem. Physiol. — 1983. — Vol. 74A, N° 2. — P. 295—299.
34. Miles P.R., Wright J.R., Bowman L., Castranova V. Incorporation of [3H]palmitate into disaturated phosphatidylcholines in alveolar type II cells isolated by centrifugal elutriation // Biochim. Biophys. Acta. — 1983. — Vol. 753, N° 1. — P. 107—118.
35. Miles P.R., Bowman L., Castranova V. Incorporation of [3H] palmitate and [l4C]choline into disaturated phosphatidylcholines in rat alveolar macrophages // Ibid. — 1985. — Vol. 833, N° 2. — P. 343—350.
36. Morrison W.R. A fast, simple and reliable method for the microdetermination of phosphor in biological material // Anal. Biochem. — 1964. — Vol. 7, N° 2. — P. 218—224.
37. Moscat J., Herrero C., Garsia-Barreno P., Municio A.M. Phospholipase C-diglyceride lipase is a major pathway for arachidonic acid release in macrophages // Biochem. Biophys. Res. Commun. — 1986. — Vol. 141, N° 1. — P. 367—373.
38. Persson A ., Chang D., Crouch E. Surfactant protein D is a divalent cation-dependent carbohydrate-binding protein // J.Biol. Chem. — 1990. — Vol. 265, № 10. — P. 5755—5760.
39. Poorthuis B.J., Yazaki P.J., Hoestetler K.Y. An improved two-dimensional thin-layer chromatography system for the separation of phosphatidylglycerol and its derivatives // J. Lipid Res. — 1976. — Vol. 17, № 4. — P. 433—437.
40. Ryan S.F., Hashim S.A., Cernansky G. et al. Quantification of surfactant phospholipids in the dog lung // Ibid. — 1980. — Vol. 21, № 8. — P. 1004—1014.
41. Shelley S.A., Paciga J.E., Balls J.M. Lung surfactant phospholipids in different animal species // Lipids. — 19S4. — Vol. 19, № 11. — P. 857—862.
42. Singh G., Katyal S.L. Surfactant apoprotein in nonmalignant pulmonary disorders // Am. J.Pathol. — 1980. — Vol. 101, № 1. — P. 51—62.
43. Smith F.B., Kikkawa Y., Diglio C.A., Dalen R.C. The type II epithelial cells of the lung. IV. Incorporation of 3H-choline and 3H-palmitate into lipids of cultured type II cells // Lab. Invest. — 1980. — Vol. 42, № 3. — P. 296—301.
44. Stern N., Riklis Sh., Kalina M., Tietz A. The catabolism of lung surfactant by alveolar macrophages // Biochim. Biophys. Acta. — 1986. — Vol. 877, № 3. — P. 323—333.
45. Stewart J.C.M. Colorimetric determination of phospholipids with ammonium ferrothiocyanate // Anal. Biochem. — 1980. — Vol. 104, № 1. — P. 10—14.
46. Tanaka Y.t Takei T. Lung surfactants. Comparison of sufractants prepared from lungs of calf, ox, dog and rabbit // Chem. Pharm Bull. — 1983. — Vol. 131, № 11. — P. 4091—4099.
47. Vaskowsky V.E., Kostetsky E.Y., Vasendin I.M. A universal reagent for phospholipid analysis // J. Chromatogr. — 1975. - Vol. 114, № 1. — P. 129—141.
48. Wagner H., Horhammer L., Wolff P. Dunnschichtchromatographie von phosphatiden und glykolipiden // Biochem. Zschr. — 1961. - Bd 334. — S. 175—184.
49. Waring A.I., Taeusch H.W. Molecular domain specific lipid-protein interactions in pulmonary surfactants containing different surfactant proteins // Am. Rev. Respir. Dis. — 1988. — Vol. 137, № 4. — Suppl. — P. A279.
50. Wright J.R., Clements J.A. Metabolism and turnover of luno surfactant // Ibid. — 1987. — Vol. 135, № 3. — P. 426—444.
51. Wright J.R. Regulation of pulmonary surfactant secreation and clearance // Ann. Rev. Rhysiol. — 1991. — Vol. 53. — P. 395—414.
52. Weaver T.E., Whitsett A. Function and regulation of expression of pulmonary surfactant-assosiated proteins // Biochem. J. - 1991. — Vol. 273. — P. 249—264.
53. Yu S.-H., Possmayer F. Reconstitution of surfactant activity by using the 6 kDa apoprotein associated with pulmonary surfactant // Ibid. — 1986. — Vol. 236, № 1. — P. 85—89.
Review
For citations:
Taganovich A.D. The biochemical methods of investigation of lung surfactant system. PULMONOLOGIYA. 1996;(2):45-50. (In Russ.)