E oxygen-dependent metabolism and synthesis of thrombocytes activation factor and different types of guinea-pigs phagocytizing cells during natural trend of experimental tuberculosis and in specific chemotherapy conditions

Abstract

Summary cell sediment (SCS) and aiveolar macrophages (AM ) pure fraction were isolated from bronchoalveolar lavage fluid (BALF) and leucocytes which were neutrophyles by 85— 89% were isolated from blood of 108 infected with tuberculosis guinea pigs in various terms of natural trend of experimental tuberculosis and after chemo therapy course. All the ceil samples were tested by spontaneous and stimulated test with nitrogen blue tetrazolinium in order to determine superoxide dismutase and catalase activity, malondialdehyde and thrombocytes activation factor (TAF) content. Eighteen healthy animals formed the control group. It was found that after in sertion of pathogen alveolar macro phages of pure fraction went to maxim umirritation , then became decompensated rapidly and then, in the terminal period of infection, they began working for self-preservation and increased the activity of antioxidant protection enzymes multiply. SCS bactericidal potential was kept longer, but AM and SCS values were equivalent in the terminal period. Blood neutrophyles bactericidal potential was kept during the whole infectious process. TAF level of SCS and neutrophyles increased sharply in infected animals, but it enlarged only for a short time in isolated AM. All the revealed changes were smoothed out in various degrees after chemotherapy course.