Investigation of nitric oxide metabolites and α1-acid glycoprotein with its glycoforms in exhaled breath condensate of bronchial asthma patients
Abstract
The aim of this study was to evaluate nitric oxide (NO) and α1-acid glycoprotein (AGP) with its glycoforms as biochemical markers of inflammatory activity in the airways of bronchial asthma (BA) patients. We examined expired breath condensate (EBC ) samples of 132 patients with severe (32) and moderate (30) BA and 30 healthy non-smoking volunteers using spectrophotometry to study NO metabolites and immunoassay to study AGP. The EBC NO metabolites levels were found to increase significantly in BA exacerbation depending on its severity. In stable BA patients the NO metabolites levels decreased but they were higher in severe and moderate BA than in healthy volunteers. The highest AGP concentration was measured in BA exacerbation with the maximal values in severe disease. In stable BA the AGP level decreased in all BA groups but it remained higher than in healthy volunteers. The qualitative structure of AGP changed during the BA exacerbation, especially in severe cases. So, according to our results the NO metabolites, AGP and its glycoforms reflect the airway inflammatory activity and can be applied to control the disease.
About the Authors
V. A. Nevzorova
Владивостокский государственный медицинский университет
Russian Federation
Russian Federation
Yu. V. Maistrovskaya
Владивостокский государственный медицинский университет
Russian Federation
Russian Federation
I. V. Chkalovets
Владивостокский государственный медицинский университет
Russian Federation
Russian Federation
K. V. Maistrovsky
Владивостокский государственный медицинский университет
Russian Federation
Russian Federation
E. A. Kochetkova
Владивостокский государственный медицинский университет
Russian Federation
Russian Federation
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Review
For citations:
Nevzorova V.A., Maistrovskaya Yu.V., Chkalovets I.V., Maistrovsky K.V., Kochetkova E.A. Investigation of nitric oxide metabolites and α1-acid glycoprotein with its glycoforms in exhaled breath condensate of bronchial asthma patients. PULMONOLOGIYA. 2003;(6):46-51. (In Russ.)
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