Metabolic support of the respiratory burst in blood and sputum neutrophils of patients with community-acquired pneumonia
https://doi.org/10.18093/0869-0189-2019-29-2-167-174
Abstract
The aim of the study was to investigate relationships between the respiratory burst in blood and sputum neutrophils and intracellular enzyme activity in patients with pneumonia. Methods. The study involved 82 patients with moderate to severe community-acquired pneumonia (CAP). The chemiluminescent assay was used to investigate synthesis of primary and secondary reactive oxygen species (ROS). The bioluminescent method was used to examine NAD(P)-dependent dehydrogenase activity in neutrophils. Results. Low-intensity respiratory burst in blood neutrophils and its predominant relations to the state of mitochondrial metabolism and malic enzyme activity were observed in healthy subjects. Blood neutrophils in patients with CAP were activated; the anaerobic respiration and mitochondrial metabolism increased in the cells. Additionally, intensity of the respiratory burst was related to terminal glycolysis reactions and the key reaction of the pentose phosphate cycle. Sputum neutrophils in patients with CAP were also activated. Conclusion. Abnormalities of metabolic support of the respiratory burst, such as decreased production of primary and secondary ROS, could appear under unfavorable conditions. Peroxidation increased in the cells, the intensity of terminal glycolysis reactions decreased, and substrates transferred from the citric acid cycle to amino acid exchange reactions. Under these conditions, malic enzyme remained the only substrate that stimulated the respiratory burst in sputum neutrophils, while substrate transfer from the tricarboxylic acid cycle inhibited synthesis of the secondary ROS.
About the Authors
Andrey A. Savchenko
Krasnoyarsk Federal Research Center of Siberian Department of Russian Academy of Medical Science; Federal Research Institute of Medical Problems of the North, Separate Unit;
V.F.Voyno-Yasenetskiy Krasnoyarsk State Medical University, Healthcare Ministry of Russia
Russian Federation
Russian Federation
Andrey A. Savchenko, Doctor of Medicine, Professor, Head of Laboratory of Cell Molecular Physiology and Pathology, Krasnoyarsk Federal Research Center of Siberian Department of Russian Academy of Medical Science, Federal Research Institute of Medical Problems of the North, Separate Unit; tel.: (391) 212-52-63
ul. Partizana Zheleznyaka 3G, Krasnoyarsk, 660022
ul. Partizana Zheleznyaka 1, Krasnoyarsk, 660022
Yuriy I. Grinshteyn
V.F.Voyno-Yasenetskiy Krasnoyarsk State Medical University, Healthcare Ministry of Russia
Russian Federation
Russian Federation
Yuriy I. Grinshteyn, Doctor of Medicine, Professor, Head of Department of Therapy, Institute of Postgraduate Medical Training, V.F.Voyno-Yasenetskiy Krasnoyarsk State Medical University, Healthcare Ministry of Russia; tel.: (391) 242-46-64
ul. Partizana Zheleznyaka 1, Krasnoyarsk, 660022
Anastasiya S. Drobysheva
I.S.Berson Krasnoyarsk Territorial Interregional Teaching Hospital No.20
Russian Federation
Russian Federation
Anastasiya S. Drobysheva, pulmonologist, .S.Berson Krasnoyarsk Territorial Interregional teaching Hospital No.20; tel.: (391) 264-29-80
ul. Instrumental’naya 12, Krasnoyarsk, 660123
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Review
For citations:
Savchenko A.A., Grinshteyn Yu.I., Drobysheva A.S. Metabolic support of the respiratory burst in blood and sputum neutrophils of patients with community-acquired pneumonia. PULMONOLOGIYA. 2019;29(2):167-174. (In Russ.) https://doi.org/10.18093/0869-0189-2019-29-2-167-174
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