pulmoПульмонологияPULMONOLOGIYA0869-01892541-9617Scientific and Practical Journal “PULMONOLOGIYA” LLC10.18093/0869-0189-2015-25-2-151-156pulmo-547Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL STUDIESЭкспрессия генов ADRB2 и CHRM3 в лейкоцитах крови у больных с обострением хронической обструктивной болезни легкихADRB2 and CHRM3 gene expression in blood leukocytes of patients with acute exacerbation of chronic obstructive pulmonary diseaseЧухловинА. Б.ChukhlovinA. B.

д. м. н., профессор, зам. директора НИИ детской онкологии, гематологии и трансплантологии им. Р.М.Горбачевой ГБОУ ВПО «Первый Санкт-Петербургский государственный медицинский университет им. акад. И.П.Павлова» Минздрава России; тел.: (812) 233-48-51;

MD, Professor, Deputy Director, R.M.Gorbacheva Scientific and Research Institute of Pediatric Oncology, Haematology and Transplantology, State Institution “Academician I.P.Pavlov First Saint-Petersburg State Medical University”, Healthcare Ministry of Russia; tel.: (812) 233-48-51;

alexei.chukh@mail.ruКузубоваН. А.KuzubovaN. A.

д. м. н., зам. директора НИИ пульмонологии ГБОУ ВПО «Первый Санкт-Петербургский государственный медицинский университет им. акад. И.П.Павлова» Минздрава России; тел.: (812) 234-17-69; 

MD, Professor, Deputy Director, Scientific and Research Pulmonology Institute, State Institution “Academician I.P.Pavlov First Saint-Petersburg State Medical University”, Healthcare Ministry of Russia; tel.: (812) 234-17-69;

kuzubova@mail.ruЕлшинН. Д.ElshinN. D.

аспирант кафедры клинической лабораторной диагностики ГБОУ ВПО «Первый Санкт-Петербургский государственный медицинский университет им. акад. И.П.Павлова» Минздрава России; тел. / факс: (812) 233-97-26;

PhD student, Department of Clinical and Laboratory Diagnostics, State Institution “Academician I.P.Pavlov First Saint-Petersburg State Medical University”, Healthcare Ministry of Russia; tel.: / fax: (812) 233-97-26;

nikita.yolshin@gmail.comТитоваО. Н.TitovaO. N.

д. м. н., директор НИИ пульмонологии ГБОУ ВПО «Первый Санкт-Петербургский государственный медицинский университет им. акад. И.П.Павлова» Минздрава России; тел.: (812) 499-68-40;

MD, Director, Scientific and Research Pulmonology Institute, State Institution “Academician I.P.Pavlov First Saint-Petersburg State Medical University”, Healthcare Ministry of Russia; tel.: (812) 499-68-40

pulmorg@mail.ruГБОУ ВПО «Первый Санкт-Петербургский государственный медицинский университет имени академика И.П.Павлова» Минздрава России: 197089, Россия, Санкт-Петербург, ул. Л.Толстого, 6 / 8РоссияState Institution “Academician I.P.Pavlov First Saint-Petersburg State Medical University”, Healthcare Ministry of Russia: 6 / 8, L'va Tolstogo ul., Saint-Petersburg, 197089, RussiaRussian Federation201524062015252151156Copyright © Чухловин А.Б., Кузубова Н.А., Елшин Н.Д., Титова О.Н., 20152015Чухловин А.Б., Кузубова Н.А., Елшин Н.Д., Титова О.Н.Chukhlovin A.B., Kuzubova N.A., Elshin N.D., Titova O.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.pulmonology.ru/pulm/article/view/547

Целью пилотного исследования явилась оценка уровней активности генов ADRB2 и CHRM3 в лейкоцитах крови у пациентов (n = 29) с обострениями хронической обструктивной болезни легких (II–III стадия, группа D по GOLD) на фоне 2-недельного комплексного лечения с применением антибактериальной терапии, тиотропия бромида, Формотерола и ингаляций глюкокортикостероидов (ГКС). До и после лечения определялось содержание мРНК генов адрено- и холинорецепторов (ADRB2 и CHRM3) в лейкоцитах крови больных. Средние значения экспрессии изученных генов достоверно не различались до и после лечения. Выявлены достоверные корреляции между изменениями экспрессии генов ADRB2 и CHRM3, динамикой объема форсированного выдоха за 1-ю секунду и уровнями С-реактивного белка в крови после купирования обострения. По результатам работы подтверждена возможность модуляции адренорецепторов под влиянием #b-агонистов и ингаляций ГКС, а также ингибирования экспрессии М-холинорецепторов при сочетанном применении ГКС и М-холинолитиков.

This pilot study was aimed at evaluation of ADRB2 and CHRM3 gene expression in COPD. Methods. We evaluated ADRB2 and CHRM3 gene expression in blood leukocytes from 29 patients with acute exacerbation of COPD (GOLD stage II to III, Group D) before and after 2-week combined therapy including antimicrobials, tiotropium bromide, formoterol and an inhaled steroid. ADRB2- and CHRM3-specific mRNA content was determined in leukocytes using real-time PCR. Results. Mean gene expression levels did not differ before and after treatment. However, we found a significant correlation between changes in ADRB2 and CHRM3 gene expression, change in FEV1 and post-treatment C-reactive protein level.

Conclusion. The results confirm a possibility to affect adrenoceptors using beta agonists and inhaled steroids and to inhibit M-cholinoreceptor expression with M3-cholinolytics and inhaled steroids.

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The authors declare that there are no conflicts of interest present.