pulmoПульмонологияPULMONOLOGIYA0869-01892541-9617Scientific and Practical Journal “PULMONOLOGIYA” LLC10.18093/0869-0189-2014-0-5-46-51pulmo-466Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL STUDIESРеспираторные вирусы и бактерии, антитела и цитокины у больных с фенотипом "бронхиальная астма и хроническая обструктивная болезнь легких"Respiratory viruses and bacteria, antibodies and cytokines in patients with bronchial asthma and chronic obstructive pulmonary disease phenotypeОспельниковаТ. П.Ospel'nikovaT. P.

к. м. н., ст. научный сотрудник лаборатории интерфероногенеза ФГБУ "НИИЭМ им. Н.Ф.Гамалеи" Минздрава России; тел.: (499) 1935582

ospelnikovat@mail.ruМорозоваО. В.MorozovaO. V.

д. б. н., вед. научный сотрудник лаборатории иммунологии ФГБУ "НИИ вирусологии им. Д.И.Ивановского" Минздрава России; тел.: (499) 1903050;

omorozova2010@gmail.comИсаеваЕ. И.IsaevaE. I.

к. б. н., вед. научный сотрудник лаборатории иммунологии ФГБУ "НИИ вирусологии им. Д.И.Ивановского" Минздрава России; тел.: (499) 1903050

immunol.lab@mail.ruОсиповаГ. Л.OsipovaG. L.

д. м. н., вед. научный сотрудник лаборатории клинической фармакологии ФГБУ "НИИ пульмонологии" ФМБА России; тел.: (499) 7800814

osipovagl@mail.ruКолодяжнаяЛ. В.KolodyazhnayaL. V.

лаборантисследователь лаборатории интерфероногенеза ФГБУ "НИИЭМ им. Н.Ф.Гамалеи" Минздрава России; тел.: (499) 1935582

ospelnikovat@mail.ruПритчинаЕ. Н.PritchinaE. N.

мл. научный сотрудник лаборатории иммунологии ФГБУ "НИИ вирусологии им. Д.И.Ивановского" Минздрава России; тел.: (499) 1903050

liza666@mail.ruСмирноваМ. Ю.SmirnovaM. Yu.

аспирант ФГБУ "НИИ пульмонологии" ФМБА России; тел.: (499) 7800814

smirnovamju@yandex.ruАндрееваС. А.AndreevaS. A.

мл. научный сотрудник лаборатории интерфероногенеза ФГБУ "НИИЭМ им. Н.Ф.Гамалеи" Минздрава России; тел.: (499) 1935582

ospelnikovat@mail.ruПанкратоваВ. Н.PankratovaV. N.

к. б. н., ст. научный сотрудник лаборатории медиаторов и эффекторов клеточного иммунитета ФГБУ "НИИЭМ им. Н.Ф.Гамалеи" Минздрава России; тел.: (499) 1935582

ospelnikovat@mail.ruЕршовФ. И.ErshovF. I.

д. м. н., профессор, академик РАН, руководитель лаборатории интерфероногенеза ФГБУ "НИИЭМ им. Н.Ф.Гамалеи" Минздрава России; тел.: (499) 1935582

felixershov@gmail.comЧучалинА. Г.ChuchalinA. G.

д. м. н., профессор, академик РАН, директор ФГБУ "НИИ пульмонологии" ФМБА России, председательправления РРО, главный внештатный специалист терапевт-пульмонолог Минздрава России; тел. / факс: (495) 4655264

chuchalin@inbox.ruФГБУ "Научно-исследовательский институт эпидемиологии и микробиологии имени почетного академика Н.Ф.Гамалеи" Минздрава России: 123098, Москва, ул. Гамалеи, 18РоссияFederal Institution "N.F.Gamaleya Research Institute", Healthcare Ministry of Russia; Moscow, RussiaRussian FederationФГБУ "Научно-исследовательский институт вирусологии им. Д.И.Ивановского" Минздрава России: 123098, Москва, ул. Гамалеи, 16РоссияFederal Institution "D.I.Ivanovskiy Research Institute", Healthcare Ministry of Russia; Moscow, RussiaRussian FederationФГБУ "НИИ пульмонологии" ФМБА России: 105077, Россия, Москва, ул. 11-я Парковая, 32, корп. 4РоссияFederal Institution "Pulmonology Research Institute", Federal Medical and Biological Agency; Moscow, RussiaRussian Federation201428102014054651Copyright © Оспельникова Т.П., Морозова О.В., Исаева Е.И., Осипова Г.Л., Колодяжная Л.В., Притчина Е.Н., Смирнова М.Ю., Андреева С.А., Панкратова В.Н., Ершов Ф.И., Чучалин А.Г., 20142014Оспельникова Т.П., Морозова О.В., Исаева Е.И., Осипова Г.Л., Колодяжная Л.В., Притчина Е.Н., Смирнова М.Ю., Андреева С.А., Панкратова В.Н., Ершов Ф.И., Чучалин А.Г.Ospel'nikova T.P., Morozova O.V., Isaeva E.I., Osipova G.L., Kolodyazhnaya L.V., Pritchina E.N., Smirnova M.Y., Andreeva S.A., Pankratova V.N., Ershov F.I., Chuchalin A.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.pulmonology.ru/pulm/article/view/466

Целью работы явился анализ респираторных вирусов и цитокинов в индуцированной мокроте больных с фенотипом "бронхиальная астма (БА) и хроническая обструктивная болезнь легких (ХОБЛ)" вне обострения и антигенов Mycoplasma pneumoniae и антител к микоплазмам и хламидиям в сыворотке крови. В образцах индуцированной мокроты у пациентов с фенотипом "БА + ХОБЛ" выявлены респираторные вирусы (риновирусы группы А, респираторносинцитиальный вирус и аденовирус), в крови – антигены микоплазм и / или антитела к M. pneumoniae и Chlamydia pneumoniae. Респираторные инфекции были ассоциированы с дисбалансом интерферонов (IFN) 1–3го типов и противовирусного белка МхА. При отсутствии экспрессии гена IFNβ в 58,3 % образцов детектировались РНК IFNα и в 42,9 % – РНК IFNλ. Показан дефицит функциональной активности IFNα и γ.

The aim of the study was to analyze respiratory viruses and cytokines in induced sputum samples, serum Mycoplasma pneumoniae antigens and antiMycoplasma and antiChlamydia antibodies in stable patients with bronchial asthma and chronic obstructive pulmonary disease (COPD) phenotype.

Methods

Methods. RNAs of rhinovirus, respiratory syncytial virus, metapneumovirus, types 1, 2, 3, and 4 influenza viruses, coronaviruses ОС43, Е229, NL63, and HKUI; DNA of group B, C and E adenoviruses and bocavirus were detected by realtime PCR with hybridization and fluorescent detection. Serum IgG, IgM and IgA antibodies against C. pneumoniae were detected with indirect microimmunofluorescence assay. Mycoplasma pneumoniae antigens were detected with aggregate hemagglutination method.

Results

Results. In patients with asthma plus COPD phenotype, three respiratory viruses were detected in sputum, such as rhinovirus, respiratory syncytial virus and adenovirus; antigens of and / or antibodies against M. pneumoniae and Chlamydia pneumoniae were also detected in blood sera. The respiratory infections were associated with imbalanced ratio of interferontypes I, II, III and MxA antiviral protein. In patients with no expression of IFNβ gene, IFNα RNA was detected in 58.3% of samples and IFNλ RNA was detected in 42.9 % of samples. Decreased functional activities of IFNα and IFNγ were found.

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The authors declare that there are no conflicts of interest present.