pulmoПульмонологияPULMONOLOGIYA0869-01892541-9617Scientific and Practical Journal “PULMONOLOGIYA” LLC10.18093/0869-0189-2014-0-3-68-72pulmo-388Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL STUDIESПОЛИМОРФИЗМ (ASP299GLY) ГЕНА TLR-4 У ВЗРОСЛЫХ БОЛЬНЫХ БРОНХИАЛЬНОЙ АСТМОЙ С АТОПИЧЕСКИМ И НЕАТОПИЧЕСКИМ ФЕНОТИПОМ В ПОПУЛЯЦИИ КРЫМАTLR-4 GENE POLYMORPHISM (ASP299GLY) IN ADULT PATIENTS WITH ATOPIC AND NON-ATOPIC BRONCHIAL ASTHMA IN THE CRIMEA POPULATIONБисюкЮ. А.BisyukYu. A.

к. м. н., доцент кафедры внутренней медицины № 2 ГУ "КГМУ им. С.И.Георгивского"; тел.: 38 (0652) 55-49-45

bisyuk@gmail.comГУ "Крымский государственный медицинский университет им. С.И.Георгиевского": 95006, Симферополь, б-р Ленина, 5 / 7РоссияState Institution S.I.Georgievskiy Crimea State Medical University; Simferopol, RussiaRussian Federation201428062014036872Copyright © Бисюк Ю.А., 20142014Бисюк Ю.А.Bisyuk Y.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.pulmonology.ru/pulm/article/view/388

Изучен полиморфизм гена TLR-4 (Asp299Gly) у пациентов с атопическим (n = 275) и неатопическим фенотипом (n = 56) бронхиальной астмы (БА). Контрольную группу составили практически здоровые жители Крыма (n = 285). Для анализа полиморфизма гена TLR-4 (Asp299Gly) был использован метод аллель-специфической полимеразной цепной реакции с электрофоретической детекцией. В контрольной группе частота распределения генотипов (AA – 85 %, AG – 14 % GG – 1 %) достоверно отличалась (c2 = 6,598, p = 0,037) от атопической БА (AA – 76 %, AG – 22 %, GG – 2 %). У пациентов с неатопической БА частота распределения генотипов (AA – 91 %, AG – 9 %, GG – 0 %) достоверно не отличалась (c2 = 1,721, p = 0,423) от контроля. Результаты исследования показали, что риск развития атопической БА связан с увеличением частоты аллеля G и уменьшением А (ОШ = 1,634, c2 = 6,08, p = 0,014) гена TLR -4. Таким образом, полиморфизм Asp299Gly гена TLR-4 связан с риском развития атопической БА в популяции Крыма. Генотип AA (Asp299 Asp) гена TLR-4 обладает протективными свойствами по отношению к развитию атопической БА. При атопической БА частота аллеля G достоверно выше, а частота аллеля A ниже по сравнению с неатопической БА.

The aim of the study was to investigate TLR-4 gene polymorphism in adult patients with atopic and non-atopic bronchial asthma in the Crimea population.Methods. TLR-4 gene polymorphism (Asp299Gly) was studied in 275 patients with atopic asthma and 56 patients with non-atopic asthma; a control group included 285 healthy residents of Crimea. The allele-specific polymerase chain reaction with electrophoretic detection of polymorphism was used.Results. In the control group, genotype frequency distribution (AA 85 %, AG 14 %, GG 1 %) was significantly different (c2 = 6.598, p = 0.037) from that in the atopic asthmatics (AA 76 %, AG 22 %, GG 2 %). In patients with non-atopic asthma, genotype frequency distribution (AA 91 %, AG 9 %, GG 0 %) did not differ significantly (c2 = 1.721, p = 0.423) from that in the control group. The results have shown that the risk of atopic asthma development was associated with increase in G-allele and decrease in A allele frequency (OR = 1.634, c2 = 6.08, p = 0.014).Conclusion. Thus, TLR-4 gene polymorphism (Asp299Gly) is related to occurrence of bronchial asthma in the Crimea population. The AA genotype (Asp299 Asp) of this gene plays a protective role against asthma occurrence. The G allele frequency in patients with atopic asthma was significantly higher than it was in patients with non-atopic asthma.

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The authors declare that there are no conflicts of interest present.