pulmoПульмонологияPULMONOLOGIYA0869-01892541-9617Scientific and Practical Journal “PULMONOLOGIYA” LLCpulmo-2511Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL STUDIESУльтраструктурные изменения в легких на фоне гипоксической терапии: оценка терапевтической эффективности как разновидности импульсной терапииAn ultrastructural observation of lungs treated by the hypoxic therapy: an assessment of its therapeutic efficacy as one of the impulse therapyОкуямаK.OkuyamaKodo

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ДжиангД.JiangJingtao

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ЛйхараК.AiharaKaoru

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Клиническая исследовательская лаборатория терапии Торным воздухом"JapanЦентральный институт электронно-микроскопических исследований, Медицинская школа НиппонJapan200417082021046771Copyright © Окуяма K., Джианг Д., Лйхара К., 20212021Окуяма K., Джианг Д., Лйхара К.Okuyama K., Jiang J., Aihara K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.pulmonology.ru/pulm/article/view/2511

Интермиттирующие гипоксические тренировки (ИГТ) используют для профилактики и лечения различных заболеваний и для тренировки спортсменов. В наш ем исследовании мы изучали морфологические изменения в легких при ИГТ с помощью световой и электронной микроскопии. 30 мужских особей крыс Wistar были подвергнуты интермиттирующим гипоксическим тренировкам (10 % 0 2) по 5 мин 3 раза с 5-минутным интервалом либо по 15, 30, 60 мин 1 раз в день в течение 21 дня. Результаты показали, что на 3-й день ИГТ возникает повреждение легких, но при кратковременной гипоксии (5 или 15 мин) через неделю эти повреждения исчезают, а в группе с 30- или 60-минутной гипоксией повреждение легких сохраняется до 21-го дня. По данным ультраструктурного исследования, в легких у животных с 5- и 15-минутной ИГТ развивается гиперплазия альвеолоцитов 2-го типа, что может способствовать более бы стром у устранению повреждений, возникающих в результате гипоксии, и это может представлять собой ключевой механизм эффекта ИГТ. Полученные данные показывают, что ИГТ можно использовать в качестве импульсной терапии.

Intermittent hypoxic training (IHT) has been used for prevention and treatment of some diseases and for training athletes. In this study, we examined the morphological changes in lungs after IHT using light and electron microscopy. 39 male Wistar rats were sustained intermittent hypoxic training (10 % 0 2) for 5 min three times a day with 5-m in rebreathing intervals or 15, 30, 60 min once a day for 21 consecutive days, respectively. Results showed that pulmonary dam age occurred at 3-rd day of IHT, but one week later the pulmonary dam age repaired in IHT animals with brief-time hypoxia (5 or 15 min), but 30-m in and 60-m in IHT groups showed pulmonary dam age until 21- st day. Ultrastructurely, lungs in 5-m in and 15-m in IHT groups were characterized by hyperplasia of alveolar type II cells, which may enable them more rapid repairing of hypoxic lesions and may represent a key mechanism of the IHT. Those findings dem onstrate that IHT could be justified as the impulse therapy for the cells and tissues.

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The authors declare that there are no conflicts of interest present.